Application
Western blot
Sample
Rat Cell lysate - whole cell (hepatocytes)
Loading amount
50 µg
Specification
hepatocytes
Gel Running Conditions
Reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/400
Incubation time
45 minute(s) · Temperature: 24°C · Diluent: TBS-milk 5%-Tween 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse alexa 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/15000
Detection
Detection method
odyssey
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 13 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Feb 18 2008