Application
Western blot
Sample
Human Cell lysate - whole cell (liver)
Loading amount
25 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Odyssey buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG-Alexa Fluor 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
Odyssey
Exposure
3 minute(s) and 10 second(s)
Bands
Specific: 14 kDa
Additional data
Additional Notes
Extraction with Triton X-100 1%. Lines represents lysates from differents hepatocytes origins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 07 2007