Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk-TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
supersignal PICO
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 70 kDa
Additional data
Abcam response
We recommend using this antibody with reduced samples. Non-reduced samples will generally appear as heavier than expected or not at all if the epitope is concealed by a non-reduced bond.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ying Chen
Verified customer
提交于 Oct 12 2007