重组Anti-Liver Arginase抗体[EPR6672(B)] (ab133543)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6672(B)] to Liver Arginase
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Liver Arginase抗体[EPR6672(B)]
参阅全部 Liver Arginase 一抗 -
描述
兔单克隆抗体[EPR6672(B)] to Liver Arginase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab217538) -
阳性对照
- WB: Human fetal liver and fetal lung lysates. IHC-P: Human hepatocellular carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6672(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)
- Anti-Liver Arginase antibody [EPR6672(B)] - BSA and Azide free (ab211961)
- Alexa Fluor® 488 Anti-Liver Arginase antibody [EPR6672(B)] (ab275992)
- Alexa Fluor® 647 Anti-Liver Arginase antibody [EPR6672(B)] (ab277922)
- Alexa Fluor® 555 Anti-Liver Arginase antibody [EPR6672(B)] (ab280848)
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Can be blocked with Liver Arginase peptide (ab217538).
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IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Can be blocked with Liver Arginase peptide (ab217538). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
靶标
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通路
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
疾病相关
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
序列相似性
Belongs to the arginase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 383 Human
- Omim: 608313 Human
- SwissProt: P05089 Human
- Unigene: 440934 Human
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别名
- A I antibody
- Al antibody
- ARG 1 antibody
see all
图片
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All lanes : Anti-Liver Arginase antibody [EPR6672(B)] (ab133543) at 1/1000 dilution
Lane 1 : Wild-type HepG2 cell lysate at 20 µg
Lane 2 : arg1 knockout HepG2 cell lysate at 20 µg
Lanes 3 & 5 : Empty at 0 µg
Lane 4 : Human Liver cell lysate at 5 µg
Lane 6 : A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Liver Arginase antibody [EPR6672(B)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133543 was shown to bind specifically to Liver Arginase. A band was observed at 36 kDa in wild-type HepG2 cell lysates with no signal observed at this size in arg1 knockout cell line ab281603 (knockout cell lysate ab282955). To generate this image, wild-type and arg1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-Liver Arginase antibody [EPR6672(B)] (ab133543) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human ARG1 protein (full-length, aa 1 to 322) (61 KDa)
Lane 2 : GST tagged Recombinant Human ARG2 protein (full-length, aa 1 to 354) (65 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)
Predicted band size: 35 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted? -
Anti-Liver Arginase antibody [EPR6672(B)] (ab133543) at 1/2000 dilution + Human liver lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Exposure time: 5 secondsBlocking and diluting buffer: 5% NFDM/TBST.
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ab133543 staining liver arginase in paraffin embedded human hepatocellular cancer tissue sections by Immunohistochemistry.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
Samples were incubated with primary antibody at 1:2000 dilution (0.13 μg/ml).
A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody.
Hematoxylin was used as a counterstain.
Cytoplasmic and nuclear staining on human hepatocellular cancer.
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Lane 1 (input): Human fetal liver whole cell lysate, 10μg
Lane 2: Human fetal liver whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133543 in Human fetal liver whole cell lysateAb133543 immunoprecipitating liver arginase in Human fetal liver whole cell lysates. Primary antibody was used at a 1:1000 dilution (0.27 μg/ml). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution. Capture antibody was used at 1:20 dilution (1.3μg in 0.35mg lysates).
Blocking and diluting buffer used: 5% NFDM/TBST.
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Tissue Microarrays stained for "Anti-Liver Arginase antibody [EPR6672(B)]” using "ab133543" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab133543 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab133543 被引用在 18 文献中.
- Laferrière F et al. Similar neuronal imprint and no cross-seeded fibrils in α-synuclein aggregates from MSA and Parkinson's disease. NPJ Parkinsons Dis 8:10 (2022). PubMed: 35027576
- Song G et al. Single-cell transcriptomic analysis suggests two molecularly subtypes of intrahepatic cholangiocarcinoma. Nat Commun 13:1642 (2022). PubMed: 35347134
- Pham TN et al. Inhibition of MNKs promotes macrophage immunosuppressive phenotype to limit CD8+ T cell antitumor immunity. JCI Insight 7:N/A (2022). PubMed: 35380995
- Huang X et al. Exosomal Circsafb2 Reshaping Tumor Environment to Promote Renal Cell Carcinoma Progression by Mediating M2 Macrophage Polarization. Front Oncol 12:808888 (2022). PubMed: 35646637
- Lo CH et al. Computational modeling reveals a key role for polarized myeloid cells in controlling osteoclast activity during bone injury repair. Sci Rep 11:6055 (2021). PubMed: 33723343