Anti-Liver Arginase抗体[ARG1/1125] (ab215843)
Key features and details
- Mouse monoclonal [ARG1/1125] to Liver Arginase
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Human
- Isotype: IgG3
概述
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产品名称
Anti-Liver Arginase抗体[ARG1/1125]
参阅全部 Liver Arginase 一抗 -
描述
小鼠单克隆抗体[ARG1/1125] to Liver Arginase -
宿主
Mouse -
经测试应用
适用于: Protein Array, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Liver Arginase aa 1-150. The exact sequence is proprietary. 87 amino acids long. NCBI Accession No. 440934.
Database link: P05089 -
阳性对照
- Human hepatocellular carcinoma tissue. 293T cells. Huma liver lysate. Recombinant Human Liver Arginase protein fragment.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
ARG1/1125 -
同种型
IgG3 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab215843于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 2 - 4 µg/ml.
Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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说明 |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2 - 4 µg/ml. Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
靶标
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通路
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
疾病相关
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
序列相似性
Belongs to the arginase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 383 Human
- Omim: 608313 Human
- SwissProt: P05089 Human
- Unigene: 440934 Human
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别名
- A I antibody
- Al antibody
- ARG 1 antibody
see all
图片
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All lanes : Anti-Liver Arginase antibody [ARG1/1125] (ab215843) at 1 µg/ml
Lane 1 : recombinant Human Liver Arginase protein fragment
Lane 2 : Human liver lysate
Predicted band size: 35 kDa -
Immunohistochemical analysis of formalix-fixed paraffin-embedded Human hepatocellular carcinoma tissue, labeling Liver Arginase using ab215843 at 4 µg/mL.
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ab215843 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
数据表及文件
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Datasheet download
文献 (0)
ab215843 尚未被引用在任何文献中。