Key features and details
- Rabbit polyclonal to LIS1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 LIS1 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human LIS1. Represents a portion of human Lissencephaly 1 (Lis 1) protein encoded in part by exons 3 and 4.
- WB: HeLa, Jurkat, K562, TCMK-1, C6 whole cell lysates
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯化说明Affinity purified using the immunising peptide immobilized on solid support.
The Abpromise guarantee
1/500 - 1/2500.
1/500 - 1/2500.
功能Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
组织特异性Fairly ubiquitous expression in both the frontal and occipital areas of the brain.
疾病相关Defects in PAFAH1B1 are the cause of lissencephaly type 1 (LIS1) [MIM:607432]; also known as classic lissencephaly. LIS1 is characterized by agyria or pachgyria and disorganization of the clear neuronal lamination of normal six-layered cortex. The cortex is abnormally thick and poorly organized with 4 primitive layers. LIS1 is associated with enlarged and dysmorphic ventricles and often hypoplasia of the corpus callosum.
Defects in PAFAH1B1 are the cause of subcortical band heterotopia (SBH) [MIM:607432]. SBH is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface.
Defects in PAFAH1B1 are a cause of Miller-Dieker lissencephaly syndrome (MDLS) [MIM:247200]. MDLS is a contiguous gene deletion syndrome of chromosome 17p13.3, characterized by classical lissencephaly and distinct facial features. Additional congenital malformations can be part of the condition.
序列相似性Belongs to the WD repeat LIS1/nudF family.
Contains 1 LisH domain.
Contains 7 WD repeats.
结构域Dimerization mediated by the LisH domain may be required to activate dynein.
细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Nucleus membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane.
- Information by UniProt
- LIS 1 antibody
- LIS 2 antibody
- LIS-1 antibody
All lanes : Anti-LIS1 antibody (ab2607) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : C6 (rat glioma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
ab2607 被引用在 15 文献中.
- Gavrilovici C et al. Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders. Cereb Cortex Commun 2:tgaa096 (2021). PubMed: 33615226
- Moon HM et al. LIS1 determines cleavage plane positioning by regulating actomyosin-mediated cell membrane contractility. Elife 9:N/A (2020). PubMed: 32159512
- Brehar FM et al. Preferential Association of Lissencephaly-1 Gene Expression with CD133+ Glioblastoma Cells. J Cancer 8:1284-1291 (2017). IHC-P ; Human . PubMed: 28607604
- Sudarov A et al. Lis1 controls dynamics of neuronal filopodia and spines to impact synaptogenesis and social behaviour. EMBO Mol Med 5:591-607 (2013). IHC-P ; Mouse . PubMed: 23483716
- Shyamasundar S et al. Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia. PLoS One 8:e65945 (2013). WB ; Mouse . PubMed: 23776576