重组Anti-LILRB1抗体[EPR22861-6] (ab238145)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22861-6] to LILRB1
- Suitable for: WB, Flow Cyt, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LILRB1抗体[EPR22861-6]
参阅全部 LILRB1 一抗 -
描述
兔单克隆抗体[EPR22861-6] to LILRB1 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt, IP, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: IM-9 whole cell lysate. ICC/IF: Human PBMC cells. Flow Cyt: Human PBMC and HEK-293T cells. IP: IM-9 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22861-6 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab238145于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 71 kDa.
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Flow Cyt |
1/500.
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IP |
1/30.
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ICC/IF |
1/50.
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说明 |
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WB
1/1000. Predicted molecular weight: 71 kDa. |
Flow Cyt
1/500. |
IP
1/30. |
ICC/IF
1/50. |
靶标
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功能
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
组织特异性
Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells. -
序列相似性
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻译后修饰
Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10859 Human
- Omim: 604811 Human
- SwissProt: Q8NHL6 Human
- Unigene: 667388 Human
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别名
- CD85 antibody
- CD85 antigen antibody
- CD85 antigen like family member J antibody
see all
图片
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All lanes : Anti-LILRB1 antibody [EPR22861-6] (ab238145) at 1/1000 dilution
Lane 1 : IM-9 (human multiple myeloma b lymphoblast) whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 71 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 22844324).
Negative control: K-562 (PMID: 22844324).
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LILRB1 was immunoprecipitated from 0.35 mg IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate with ab238145 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238145 at a 1/1000 dilution (0.48 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10μg
Lane 2: ab238145 IP in IM-9 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238145 in IM-9 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling LILRB1 with ab238145 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of human PBMC is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged LILRB2 expression vector (Left) / HEK-293T transfected with myc-tagged LILRB1 expression vector (Right) cells labeling LILRB1 with ab238145 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG (black) or ab238145 (red). Then fixed with 2% PFA followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647. Gated on myc+ population.
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling LILRB1 with ab238145 at 1/500 dilution (0.1μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with anti-CD11b conjugated to BV421. Then stained with rabbit IgG (Left) or ab238145 (Right). Gated on viable cells.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab238145 被引用在 2 文献中.
- Jiao Y et al. Effect of remote limb ischemic post-conditioning on the expression of miR-21-5p/PirB in the brain of rats with focal cerebral ischemia. Eur J Neurosci 55:1105-1117 (2022). PubMed: 35060207
- Zhang Y et al. Poor Prognosis and Therapeutic Responses in LILRB1-Expressing M2 Macrophages-Enriched Gastric Cancer Patients. Front Oncol 11:668707 (2021). PubMed: 34485116