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Our Abpromise guarantee covers the use of ab84571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 5 µg/ml.|
ab84571 staining LGN in Dog kidney cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C.
ab84571 at 5 µg/ml staining LGN in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab84571 at 5 µg/ml staining LGN in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab84571 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"