重组Anti-LGALS14抗体[EPR9665] (ab150427)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9665] to LGALS14
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-LGALS14抗体[EPR9665]
参阅全部 LGALS14 一抗 -
描述
兔单克隆抗体[EPR9665] to LGALS14 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human LGALS14 aa 100-200. The exact sequence is proprietary.
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阳性对照
- Recombinant Human LGALS14 protein (ab101087) can be used as a positive control in WB. Human placenta lysate; Mouse placenta lysate; JAR cells. ICC/IF: JAR cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9665 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150427于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/20.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/20. |
靶标
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功能
Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis. -
组织特异性
Highly expressed in placenta. -
序列相似性
Contains 1 galectin domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 56891 Human
- Omim: 607260 Human
- SwissProt: Q8TCE9 Human
- Unigene: 24236 Human
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别名
- Charcot Leyden crystal protein 2 antibody
- Charcot-Leyden crystal protein 2 antibody
- CLC2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution (Purified) + Human placenta lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa -
Intracellular Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Human placenta lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Mouse placenta lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
Immunofluorescent analysis of JAR cells labelling LGALS14 with unpurified ab150427 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab150427 被引用在 1 文献中.
- Wang M et al. Galectin-14 Promotes Trophoblast Migration and Invasion by Upregulating the Expression of MMP-9 and N-Cadherin. Front Cell Dev Biol 9:645658 (2021). PubMed: 33796532