重组Anti-Leukotriene A4 hydrolase/LTA4H抗体[EPR5713] (ab133512)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5713] to Leukotriene A4 hydrolase/LTA4H
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Leukotriene A4 hydrolase/LTA4H抗体[EPR5713]
参阅全部 Leukotriene A4 hydrolase/LTA4H 一抗 -
描述
兔单克隆抗体[EPR5713] to Leukotriene A4 hydrolase/LTA4H -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Leukotriene A4 hydrolase/LTA4H aa 550-650. The exact sequence is proprietary.
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阳性对照
- WB: HeLa, 293T, T47D and A549 cell lysates. IHC-P: Human kidney tissue. Flow Cyt (intra): Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 5.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR5713 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 69 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity. -
通路
Lipid metabolism; leukotriene B4 biosynthesis. -
序列相似性
Belongs to the peptidase M1 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4048 Human
- Entrez Gene: 16993 Mouse
- Entrez Gene: 299732 Rat
- Omim: 151570 Human
- SwissProt: P09960 Human
- SwissProt: P24527 Mouse
- SwissProt: P30349 Rat
- Unigene: 524648 Human
see all -
别名
- FLJ17564 antibody
- Leukotriene A(4) hydrolase antibody
- Leukotriene A-4 hydrolase antibody
see all
图片
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All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : LTA4H knockout HEK293T cell lysate
Lane 3 : T-47D cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDaLanes 1-4: Merged signal (red and green). Green - ab133512 observed at 69 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133512 Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] was shown to specifically react with Leukotriene in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266467 (knockout cell lysate ab258034) was used. Wild-type and Leukotriene knockout samples were subjected to SDS-PAGE. ab133512 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Overlay histogram showing Jurkat cells stained with ab133512 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133512, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Leukotriene A4 hydrolase/LTA4H with ab133512 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : T47D cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa -
All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5713] (ab133512) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab133512 被引用在 7 文献中.
- Miek L et al. Staphylococcus aureus controls eicosanoid and specialized pro-resolving mediator production via lipoteichoic acid. Immunology 166:47-67 (2022). PubMed: 35143048
- Zhang K et al. Modulation of Inflammation-Related Lipid Mediator Pathways by Celastrol During Human Macrophage Polarization. J Inflamm Res 15:3285-3304 (2022). PubMed: 35676971
- Chan SJ et al. Early and Sustained Increases in Leukotriene B4 Levels Are Associated with Poor Clinical Outcome in Ischemic Stroke Patients. Neurotherapeutics 17:282-293 (2020). PubMed: 31520306
- Werner M et al. Targeting biosynthetic networks of the proinflammatory and proresolving lipid metabolome. FASEB J 33:6140-6153 (2019). PubMed: 30735438
- Tsuda S et al. Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B4 pathway by high-density lipoprotein in macrophages. Sci Rep 7:12989 (2017). PubMed: 29021582
- Lin HN & O'Connor JP Immunohistochemical localization of key arachidonic acid metabolism enzymes during fracture healing in mice. PLoS One 9:e88423 (2014). IHC-P ; Mouse . PubMed: 24516658
- Dixit N et al. Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis. Arthritis Res Ther 16:496 (2014). ICC/IF ; Human . PubMed: 25443625