Anti-LeuRS抗体(ab31534)
Key features and details
- Rabbit polyclonal to LeuRS
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LeuRS抗体
参阅全部 LeuRS 一抗 -
描述
兔多克隆抗体to LeuRS -
宿主
Rabbit -
特异性
This antibody reacts specifically with LeuRS.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31534于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000. Predicted molecular weight: 135 kDa.
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IHC-P |
1/200.
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说明 |
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WB
1/1000. Predicted molecular weight: 135 kDa. |
IHC-P
1/200. |
靶标
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功能
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs. -
疾病相关
Infantile liver failure syndrome 1 -
序列相似性
Belongs to the class-I aminoacyl-tRNA synthetase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 51520 Human
- Omim: 151350 Human
- SwissProt: Q9P2J5 Human
- Unigene: 432674 Human
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别名
- cytoplasmic antibody
- Cytoplasmic leucyl tRNA synthetase antibody
- Cytosolic leucyl tRNA synthetase antibody
see all
图片
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All lanes : Anti-LeuRS antibody (ab31534) at 1/4000 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDa -
ab31534 used in Western Blot at a 1/4,000 dilution.
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ab31534 at a 1/200 dilution staining LeuRS in human colon cancer tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab31534 被引用在 8 文献中.
- Fu S et al. HIV-1 exploits the Fanconi anemia pathway for viral DNA integration. Cell Rep 39:110840 (2022). PubMed: 35613597
- He XD et al. Sensing and Transmitting Intracellular Amino Acid Signals through Reversible Lysine Aminoacylations. Cell Metab 27:151-166.e6 (2018). PubMed: 29198988
- Su X et al. Interferon-? regulates cellular metabolism and mRNA translation to potentiate macrophage activation. Nat Immunol 16:838-849 (2015). PubMed: 26147685
- Park SJ et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . PubMed: 26544075
- Carlin MB et al. Skeletal muscle Ras-related GTP binding B mRNA and protein expression is increased after essential amino acid ingestion in healthy humans. J Nutr 144:1409-14 (2014). PubMed: 25056691
- Guan BJ et al. Translational control during endoplasmic reticulum stress beyond phosphorylation of the translation initiation factor eIF2a. J Biol Chem 289:12593-611 (2014). WB ; Mouse . PubMed: 24648524
- Gulati P et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB ; Mouse . PubMed: 23359686
- Shin SH et al. Implication of leucyl-tRNA synthetase 1 (LARS1) over-expression in growth and migration of lung cancer cells detected by siRNA targeted knock-down analysis. Exp Mol Med 40:229-36 (2008). WB ; Human . PubMed: 18446061