Anti-Lethal factor抗体[5F502.2] (ab13814)
Key features and details
- Mouse monoclonal [5F502.2] to Lethal factor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Lethal factor抗体[5F502.2]
参阅全部 Lethal factor 一抗 -
描述
小鼠单克隆抗体[5F502.2] to Lethal factor -
宿主
Mouse -
特异性
The antibody has been tested against recombinant protein only (0.1 ug recombinant protein was loaded per lane). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Lethal factor.
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阳性对照
- 0.1 ug recombinant protein
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
5F502.2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13814于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 90 kDa.
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说明 |
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WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 90 kDa. |
靶标
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相关性
The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates. -
细胞定位
secreted -
别名
- Anthrax lethal toxin endopeptidase component antibody
- Anthrax LF antibody
- Bacillus anthracis lethal factor antibody
see all
数据表及文件
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Datasheet download
文献 (2)
ab13814 被引用在 2 文献中.
- Kim SK et al. Comparative analysis of virulence factors secreted by Bacillus anthracis Sterne at host body temperature. Lett Appl Microbiol 54:306-12 (2012). PubMed: 22268495
- Geddie ML et al. Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors. J Biol Chem 280:35641-6 (2005). PubMed: 16118206