概述

性能

应用

Our Abpromise guarantee covers the use of ab9749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mouse Leptin (using 100 µl/well antibody solution).
WB Use a concentration of 0.1 - 0.2 µg/ml.
IHC-P Use a concentration of 0.2 µg/ml.

靶标

  • 功能
    May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • 疾病相关
    Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性
    Belongs to the leptin family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

图片

  • ab9749 staining Leptin in murine cecum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in 100mM citric acid. The primary antibody was used at 0.20 ug/ml and incubated with sample at 4°C overnight. A cyanine-3 conjugated donkey polyclonal to rabbit was used as secondary.

文献

This product has been referenced in:
  • de Oliveira M  et al. Triiodothyronine Increases mRNA and Protein Leptin Levels in Short Time in 3T3-L1 Adipocytes by PI3K Pathway Activation. PLoS One 8:e74856 (2013). Read more (PubMed: 24058635) »
See 1 Publication for this product

客户评价及客户问答

1-2 of 2 Q&A

Question

Thank you for your reply. Sorry, I wrote the antibody code wrong. I've purshased the anti-ghrelin receptor antibody -ab85104. So, could you tell me which form of the receptor this antibody recognizes? Active or truncated form? If you have any reference on this, I would appreciate if you could send to me.
Regarding anti-leptin receptor, I thought very strange that we could use it on only 6 glass slides! And now it is almost over! Does it usually come in so low ammount? This is very diffent from all other antibodies we use here at the lab. We usually can use the antibody in many reactions!
We are studying human tissue in parraffin blocks. There are no other studies with anti-leptin receptor on the tumor we are investigating (neurofibromas). I am sure if we could finish this research, it would gave us the opportunity to publish the results in a good scientic journal. And I think this is going to be good for Abcam, since there are no studies using this antibody in the literature, even with other samples. At least I could not find them.
I understand you carry over thousands of products, but I would like to ask again if would be possible to send us a sample of anti-ghrelin receptor. We waited for so long to receive this antibody here in Brazil and because of taxes, we paid much more for it: $ 1.160,00! This antibody is going to be used in a thesis of a student of mine and now we do not know what to do. We cannot pay again too much for another antibody to use on only 6 glass slides!
I am sorry to tell all those things, but I hope Abcam can help us. Publishing these results will be important for us and I think it would also be good for Abcam.
Thank you very much

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Answer

Thank you for your reply.
Unfortunately, we haven't tested if this ab85104 Anti-Ghrelin Receptor antibody can distinguish between the GHS-R1a and GHS-R1b protein. However, as the immunogen is derived from the conserved N-terminus of both forms, it is possible that the antibody cannot distinguish those receptors, and therefore, detects both forms.
Regarding the ab9749 Anti-Leptin antibody, I am wondering if you have purchased this product over one of our official distributors Biogen or Sellex? Do you remember if you received 200ul? Indeed the price seems very high, and I am surprised that you were only able to use it on 6 slides out of a 200ul product (seen that you dilute this antibody approximately at 1/250).
In addition, it looks like that this Leptin antibody has only been tested on mouse so far, but not on human samples. This, unfortunately, means that we cannot guarantee that the antibody will be working on human samples in the first place. We can see whether we could provide you with a testing discount as we do not carry any test samples of our antibodies.
However, we need the additional information we are requesting from you and you would need to contact then our official distributor.
The ab85104 Anti-Ghrelin Receptor antibody is already tested on both application and species and therefore not appropriate for this offer.
I am looking forward to your reply.

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Answer

Thank you for contacting us.
According to SwissProt, O-octanoylation or O-decanoylation is essential for Ghrelin activity. As this antibody detects both the octanoylated and non-octanoylated forms of Ghrelin, the antibody detects therefore both the active and form of the hormone. However, I am wondering if you are referring to the Ghrelin Receptors and not to the hormone? If this is the case, this antibody is unsuitable for you.
Regarding the Leptin antibody, because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. Nonetheless, may I ask what kind of species you are working on?
I am looking forward to your reply.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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