重组
RabMAb

Anti-LDL Receptor抗体[EP1553Y] (ab52818)

概述

  • 产品名称
    Anti-LDL Receptor抗体[EP1553Y]
    参阅全部 LDL Receptor 一抗
  • 描述
    兔单克隆抗体[EP1553Y] to LDL Receptor
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide within Human LDL Receptor (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • HepG2 cells and cell lysate
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

应用

Our Abpromise guarantee covers the use of ab52818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
IHC-P 1/500.

See IHC antigen retrieval protocol.

WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Flow Cyt 1/70 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
  • 疾病相关
    Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
  • 序列相似性
    Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • 翻译后修饰
    N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.
  • 细胞定位
    Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FH antibody
    • FHC antibody
    • LDL R antibody
    • LDL receptor antibody
    • LDLCQ2 antibody
    • Ldlr antibody
    • LDLR_HUMAN antibody
    • Low Density Lipoprotein Receptor antibody
    • Low density lipoprotein receptor class A domain containing protein 3 antibody
    • Low density lipoprotein receptor familial hypercholesterolemia antibody
    • Low-density lipoprotein receptor antibody
    see all

图片

  • All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
    Lane 3 : HeLa treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
    Lane 4 : Raw264.7 whole cell lysates
    Lane 5 : Raw264.7 treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
    Lane 6 : Raw264.7 treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

    Predicted band size: 95 kDa
    Observed band size: 140 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute


    Blocking buffer: 5% NFDM/TBST

    Diltuion buffer: 5% NFDM/TBST

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LDL Receptor with purified ab52818 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor®594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

    For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) were used. 

  • Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 0.4 µg/ml + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 95 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with the literature (PMID: 15199428, PMID: 8349823, PMID: 10906332, PMID: 24918045).

  • Immunohistochemical analysis of paraffin-embedded human liver sections labellingLDL Receptor with purified ab52818 at dilution of 1:500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Overlay histogram showing 4% paraformaldehyde fixed HeLa cells labelling LDL Receptor (red) with purified ab52818 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG at dilution of 1/2000. A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

    Lane 1 : mouse liver lysate
    Lane 2 : mouse lung lysate
    Lane 3 : mouse colon lysate
    Lane 4 : human liver lysate
    Lane 5 : HepG2 cell lysate
    Lane 6 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (HRP goat anti-rabbit IgG (H+L))

    Predicted band size: 95 kDa
    Observed band size: 140 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST

    Diltuion buffer: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/5000 dilution + Mouse liver at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 95 kDa
    Observed band size: 140 kDa (why is the actual band size different from the predicted?)



    Blocking/Diluting buffer 5% NFDM/TBST
  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • ICC/IF image of ab52818 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52818, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing U937 cells stained with ab52818 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52818, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 95 kDa
    Observed band size: 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    LDL Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

文献

This product has been referenced in:
  • Zhu L  et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Mol Metab 8:106-116 (2018). WB . Read more (PubMed: 29331506) »
  • Xie Z  et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29502100) »

See all 42 Publications for this product

客户评价及客户问答

Gracias por tu respuesta.

Acabo de crear un código de descuento para testar ab52818 mediante Western Blot con lisados de células de conejo.

El código es: ABTRIAL-EBDAM. Caduca el día 23-Mayo-2013.
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Thanks for your email.
I believe this replacement order has been shipped and delivered on Tuesday July 18. It was on Abcam order reference number 1128916 which contained a vial of ab61392.
I hope this is helpful. Please let me know if you hav...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab61392 and one vial of ab52818.

To check the status of th...

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Thank you for sending along the additional PO information. I have located that order for ab61392 on our system.
I am happy to send out the replacement antibodies. Since a couple options have been discussed for these replacement antibodies, I just ...

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Thanks for your reply.
Again I apologize for the trouble finding antibodies to 3-nitrotyrosine & the human LDL receptor. The antibodies you have ordered so far have very few complaints and are ordered relatively frequently so it is certainly an un...

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Thanks for sending along the purchase order information.
Based on our records, you received a replacement end of May for ab30532. This replacement antibody was ab52818. So I can send you 1 free antibody to replace vial of ab52818 that did not work...

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Thank you for contacting Abcam. The epitope for this antibody is within the *********of the protein, please see the link below for more information about the structure of the protein: http://www.uniprot.org/uniprot/P01130 Also, as I mentioned on the ph...

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Thank you for contacting us. The product ab52818 is raised against the C-terminus, which is the intracellular region. Therefore permeabilization will be required for cell staining. ab30532 is raised against an immunogen corresponding to amino a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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