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Immunology Adaptive Immunity T Cells CD

Anti-Lck抗体(ab3885)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (6)

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Western blot - Anti-Lck antibody (ab3885)
  • Western blot - Anti-Lck antibody (ab3885)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody (ab3885)

Key features and details

  • Rabbit polyclonal to Lck
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Lck抗体
    参阅全部 Lck 一抗
  • 描述

    兔多克隆抗体to Lck
  • 宿主

    Rabbit
  • 特异性

    This antibody was designed to recognise the activated form of Lck and was raised for that purpose against the linker that connects the SH2 domain and the tyrosine kinase domain of the kinase. This linker sits in the SH3 substrate binding site in the inactive kinase. The position of the linker is based on the crystal structure of inactive Src. However, the antibody does not seem to distinguish the active and inactive forms of Lck. In IF, it recognises kinase dead Lck at the same concentration as constitutively active Lck. In western blot, the antibody is highly specific for Lck versus other tyrosine kinases (see blot). Even, if the antibody was specific for active Lck in IF , it would be expected to recognise both active and inactive forms in Western blot. This is because the linker that the antibody recognises is present in both active and inactive Lck, it was just expected to be concealed in native form inactive Lck.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Human
    African green monkey
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human Lck aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13750)

  • 常规说明


    Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Immunology
    • Secreted Molecules
    • Other secreted molecules

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Lck protein (ab79626)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Mouse
WB
Human
African green monkey
All applications
Chicken
应用 Ab评论 说明
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF
1/200 - 1/500.
说明
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF
1/200 - 1/500.

靶标

  • 功能

    Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
  • 组织特异性

    Expressed specifically in lymphoid cells.
  • 疾病相关

    Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 结构域

    The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
  • 翻译后修饰

    Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
  • 细胞定位

    Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
  • Target information above from: UniProt accession P06239 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 396460 Chicken
    • Entrez Gene: 3932 Human
    • Entrez Gene: 16818 Mouse
    • Omim: 153390 Human
    • SwissProt: P42683 Chicken
    • SwissProt: P06239 Human
    • SwissProt: P06240 Mouse
    • Unigene: 470627 Human
    • 形式

      This protein is known to be similar in amino acid sequence to HCK (P08631), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • 别名

      • IMD22 antibody
      • LCK antibody
      • Lck p56 antibody
      • LCK proto-oncogene, Src family tyrosine kinase antibody
      • LCK_HUMAN antibody
      • Leukocyte C-terminal Src kinase antibody
      • LSK antibody
      • Lymphocyte cell specific protein tyrosine kinase antibody
      • Lymphocyte cell-specific protein-tyrosine kinase antibody
      • Lymphocyte specific protein tyrosine kinase antibody
      • Membrane associated protein tyrosine kinase antibody
      • Oncogene lck antibody
      • P56 LCK antibody
      • p56(LSTRA) protein tyrosine kinase antibody
      • p56-LCK antibody
      • p56lck antibody
      • pp58 lck antibody
      • pp58lck antibody
      • Protein YT16 antibody
      • Proto oncogene tyrosine protein kinase LCK antibody
      • Proto-oncogene Lck antibody
      • Protooncogene tyrosine protein kinase LCK antibody
      • T cell specific protein tyrosine kinase antibody
      • T cell-specific protein-tyrosine kinase antibody
      • T lymphocyte specific protein tyrosine kinase p56lck antibody
      • Tyrosine-protein kinase Lck antibody
      • YT 16 antibody
      • YT16 antibody
      see all

    图片

    • Western blot - Anti-Lck antibody (ab3885)
      Western blot - Anti-Lck antibody (ab3885)
      All lanes : Anti-Lck antibody (ab3885) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : Jurkat nuclear extract lysate (ab14844)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 57 kDa
      Observed band size: 57 kDa


      Exposure time: 4 minutes
    • Western blot - Anti-Lck antibody (ab3885)
      Western blot - Anti-Lck antibody (ab3885)
      All lanes : Anti-Lck antibody (ab3885) at 1/1000 dilution

      Lane 1 : COS cells transfected with mouse Fyn
      Lane 2 : COS cells transfected with mouse Lck

      Predicted band size: 57 kDa



      Western blot against Lck using ab3885.

      Lane 1: COS cells transfected with mouse Fyn.

      Lane 2: COS cells transfected with mouse Lck.

    • Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody (ab3885)
      Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody (ab3885)

      Confocal immunofluorescence image of a COS cell transfected with a mouse Lck construct and stained with ab3885 at 1/200.

      In untransfected cells, some nuclear staining is also visible as well as Lck staining.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab3885?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3885 被引用在 6 文献中.

    • Lamm KYB  et al. Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome. Elife 7:N/A (2018). PubMed: 29309034
    • Singh DK  et al. SRC family kinases in hamster spermatozoa: evidence for the presence of LCK. Reproduction 153:655-669 (2017). PubMed: 28250239
    • Luo T  et al. Lck Inhibits Heat Shock Protein 65-Mediated Reverse Cholesterol Transport in T Cells. J Immunol 197:3861-3870 (2016). PubMed: 27742830
    • Cao W  et al. CXXC finger protein 1 is critical for T-cell intrathymic development through regulating H3K4 trimethylation. Nat Commun 7:11687 (2016). PubMed: 27210293
    • Clayton KL  et al. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol 192:782-91 (2014). WB . PubMed: 24337741
    • Rüder C  et al. The tumor-associated antigen EBAG9 negatively regulates the cytolytic capacity of mouse CD8+ T cells. J Clin Invest 119:2184-203 (2009). IHC (PFA fixed) ; Mouse . PubMed: 19620783

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lck antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (thymus)
    Specification
    thymus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    提交于 Apr 07 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lck antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (thymus)
    Specification
    thymus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    提交于 Mar 26 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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