Key features and details
- Rabbit polyclonal to Lck
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
参阅全部 Lck 一抗
特异性This antibody was designed to recognise the activated form of Lck and was raised for that purpose against the linker that connects the SH2 domain and the tyrosine kinase domain of the kinase. This linker sits in the SH3 substrate binding site in the inactive kinase. The position of the linker is based on the crystal structure of inactive Src. However, the antibody does not seem to distinguish the active and inactive forms of Lck. In IF, it recognises kinase dead Lck at the same concentration as constitutively active Lck. In western blot, the antibody is highly specific for Lck versus other tyrosine kinases (see blot). Even, if the antibody was specific for active Lck in IF , it would be expected to recognise both active and inactive forms in Western blot. This is because the linker that the antibody recognises is present in both active and inactive Lck, it was just expected to be concealed in native form inactive Lck.
经测试应用适用于: WB, ICC/IFmore details
种属反应性与反应: Mouse, Human, African green monkey
Synthetic peptide corresponding to Human Lck aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available as
Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells.
Our Abpromise guarantee covers the use of ab3885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|ICC/IF||1/200 - 1/500.|
功能Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
组织特异性Expressed specifically in lymphoid cells.
疾病相关Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
结构域The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
翻译后修饰Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
细胞定位Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
- Information by UniProt
形式This protein is known to be similar in amino acid sequence to HCK (P08631), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
- IMD22 antibody
- LCK antibody
- Lck p56 antibody
All lanes : Anti-Lck antibody (ab3885) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 4 minutes
All lanes : Anti-Lck antibody (ab3885) at 1/1000 dilution
Lane 1 : COS cells transfected with mouse Fyn
Lane 2 : COS cells transfected with mouse Lck
Predicted band size: 57 kDa
Western blot against Lck using ab3885.
Lane 1: COS cells transfected with mouse Fyn.
Lane 2: COS cells transfected with mouse Lck.
Confocal immunofluorescence image of a COS cell transfected with a mouse Lck construct and stained with ab3885 at 1/200.
In untransfected cells, some nuclear staining is also visible as well as Lck staining.
ab3885 被引用在 6 文献中.
- Lamm KYB et al. Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome. Elife 7:N/A (2018). PubMed: 29309034
- Singh DK et al. SRC family kinases in hamster spermatozoa: evidence for the presence of LCK. Reproduction 153:655-669 (2017). PubMed: 28250239
- Luo T et al. Lck Inhibits Heat Shock Protein 65-Mediated Reverse Cholesterol Transport in T Cells. J Immunol 197:3861-3870 (2016). PubMed: 27742830
- Cao W et al. CXXC finger protein 1 is critical for T-cell intrathymic development through regulating H3K4 trimethylation. Nat Commun 7:11687 (2016). PubMed: 27210293
- Clayton KL et al. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol 192:782-91 (2014). WB . PubMed: 24337741
- Rüder C et al. The tumor-associated antigen EBAG9 negatively regulates the cytolytic capacity of mouse CD8+ T cells. J Clin Invest 119:2184-203 (2009). IHC (PFA fixed) ; Mouse . PubMed: 19620783