重组Anti-LC3C抗体[EPR8076] (ab167421)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8076] to LC3C
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-LC3C抗体[EPR8076]
参阅全部 LC3C 一抗 -
描述
兔单克隆抗体[EPR8076] to LC3C -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human LC3C.
-
阳性对照
- LC3C transfected 293T cell lysates, paraffin-embedded Human lung tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8076 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab167421于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
组织特异性
Most abundant in placenta, lung and ovary. -
序列相似性
Belongs to the MAP1 LC3 family. -
翻译后修饰
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
细胞定位
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
-
数据库链接
- Entrez Gene: 440738 Human
- Omim: 609605 Human
- SwissProt: Q9BXW4 Human
- Unigene: 534971 Human
-
别名
- ATG8J antibody
- Autophagy-related protein LC3 C antibody
- Autophagy-related ubiquitin-like modifier LC3 C antibody
see all
图片
-
All lanes : Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution
Lane 1 : LC3C transfected 293T cell lysate
Lane 2 : Mock transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab167421 尚未被引用在任何文献中。