Application
Western blot
Sample
Rat Cell lysate - whole cell (rat primary cortical neuronal cultures)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Treatment
treated or not treated with Bafilomycin A1 (BAF) 300nM for 6h
Specification
rat primary cortical neuronal cultures
Blocking step
casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in bloking solution (casein 0.1%)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit IgG from LiCOR (IRDye 800)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey Infrared Imaging System
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 16, 14 kDa
Additional data
Additional Notes
Rat primary cortical neuronal cultures treated or not treated with Bafilomycin A1 (BAF) 300nM for 6h.
Whereas the ab48934 displayed the expected bands for either LC3-I (16kDa) and LC3-II (14kDa), the ab232940 did not seem to recognize the LC3-II band.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 08 2019