Anti-LAT抗体(ab2507)
Key features and details
- Rabbit polyclonal to LAT
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-LAT抗体
参阅全部 LAT 一抗 -
描述
兔多克隆抗体to LAT -
宿主
Rabbit -
特异性
Binds to a 36-40 Kd protein in normal mouse lung and heart tissue. A larger size band of approx. 40-45 Kd is seen in human tumour cell lines. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide:
CHRLPGSYDSTSSDSLYPRGI
, corresponding to amino acids 29-48 of Human LAT.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. |
靶标
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功能
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. -
组织特异性
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). -
翻译后修饰
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. -
细胞定位
Cell membrane. Present in lipid rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 27040 Human
- Entrez Gene: 16797 Mouse
- Omim: 602354 Human
- SwissProt: O43561 Human
- SwissProt: O54957 Mouse
- Unigene: 632179 Human
- Unigene: 10280 Mouse
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别名
- 36 kDa phospho tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adaptor protein antibody
see all
图片
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Western blot - Anti-LAT antibody (ab2507)Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).
Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).
文献 (4)
ab2507 被引用在 4 文献中.
- Kota DJ et al. TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity. Diabetes 62:2048-58 (2013). WB . PubMed: 23349496
- Zikherman J et al. Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival. Proc Natl Acad Sci U S A 109:E3-12 (2012). WB ; Mouse . PubMed: 22135465
- Levin SE et al. Inhibition of ZAP-70 Kinase Activity via an Analog-sensitive Allele Blocks T Cell Receptor and CD28 Superagonist Signaling. J Biol Chem 283:15419-15430 (2008). WB ; Human . PubMed: 18378687
- Head BP et al. Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components. J Biol Chem 281:26391-9 (2006). PubMed: 16818493
