Key features and details
- Goat polyclonal to LASP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
参阅全部 LASP1 一抗
经测试应用适用于: WBmore details
预测可用于: Mouse, Rat, Chicken, Pig
- WB: Recombinant Human LASP1 protein (ab114888), Mouse brain extracts.
常规说明Principal Names: LASP1 (LIM and SH3 protein 1), MLN50. GenBank Accession Number: NP006139 LocusLink ID: 3927 Please note that one of our customers has had problems getting this antibody to detect endogenous Lasp-1 in 2 different human cell lines. Furthermore, a LASP-1/GFP fusion protein overexpressed in a cell line could not be detected with this antibody and yet was detected with our Rabbit Polyclonal to GFP (ab290). For further information please see the enquiries section of the online datasheet. We are currently conducting more QC tests on this product and are interested to hear from other researchers using it. 24 March, 2004.
This protein functions as an actin-binding protein and possibly in cytoskeletal organization.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody说明This protein functions as an actin-binding protein and possibly in cytoskeletal organization.
Our Abpromise guarantee covers the use of ab1301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
1 hour primary incubation is recommended for this product.
功能Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
序列相似性Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain.
细胞定位Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
- Information by UniProt
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
LASP1 staining (0.5
µg/ml) of Mouse Brain extracts (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence LASP1 staining (0.5µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence
ab1301 被引用在 1 文献中.
- Li K et al. Organelle proteomics of rat synaptic proteins: correlation-profiling by isotope-coded affinity tagging in conjunction with liquid chromatography-tandem mass spectrometry to reveal post-synaptic density specific proteins. J Proteome Res 4:725-33 (2005). PubMed: 15952719