Anti-LAP2 alpha抗体(ab5162)
Key features and details
- Rabbit polyclonal to LAP2 alpha
- Suitable for: IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-LAP2 alpha抗体
参阅全部 LAP2 alpha 一抗 -
描述
兔多克隆抗体to LAP2 alpha -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human LAP2 alpha aa 188-693.
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常规说明
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: PBS, Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
Primary antibody说明
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
|
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| WB | (1) |
1/2500. Detects a band of approximately 83 kDa.
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/2500. Detects a band of approximately 83 kDa. |
靶标
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相关性
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 7112 Human
- Entrez Gene: 21917 Mouse
- Entrez Gene: 25359 Rat
- Omim: 188380 Human
- SwissProt: P42166 Human
- SwissProt: Q61033 Mouse
- SwissProt: Q62733 Rat
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别名
- Isoforms beta/gamma antibody
- Lamina associated polypeptide 2 antibody
- LAP2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody (ab5162)
ab5162 at a dilution of 1/250 staining LAP 2 alpha in HeLa cells by immunofluorescence.
A = ab5162, 1/250
B = DNA
C = A and B merged
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Western blot - Anti-LAP2 alpha antibody (ab5162)ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL). ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab5162 被引用在 17 文献中.
- Chojnowski A et al. Heterochromatin loss as a determinant of progerin-induced DNA damage in Hutchinson-Gilford Progeria. Aging Cell 19:e13108 (2020). PubMed: 32087607
- Nastaly P et al. Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus. Nat Commun 11:2122 (2020). PubMed: 32358486
- Gao N et al. A Role of Lamin A/C in Preventing Neuromuscular Junction Decline in Mice. J Neurosci 40:7203-7215 (2020). PubMed: 32817327
- Mirza AN et al. LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription. Cell 176:198-212.e15 (2019). PubMed: 30503211
- Qin Z et al. Long noncoding RNA TMPO-AS1 promotes progression of non-small cell lung cancer through regulating its natural antisense transcript TMPO. Biochem Biophys Res Commun 516:486-493 (2019). PubMed: 31230752