概述

  • 产品名称

    Anti-LAMP2抗体[GL2A7]
    参阅全部 LAMP2 一抗
  • 描述

    大鼠单克隆抗体[GL2A7] to LAMP2
  • 宿主

    Rat
  • 经测试应用

    适用于: Flow Cyt, IHC-P, IHC-Fr, IP, ICC, ICC/IF, WB, IHC-FoFrmore details
  • 种属反应性

    与反应: Mouse, Rabbit, Human
  • 免疫原

    Tissue, cells or virus corresponding to Mouse LAMP2.

  • 阳性对照

    • WB: HEK-293 cell lysate. ICC/IF: Adult mouse cardiac myocytes; rabbit corneal endothelial cells. IHC-Fr: Mouse uterus tissue.
  • 常规说明

    For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

性能

应用

Our Abpromise guarantee covers the use of ab13524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. (PMID 2142158).
ICC 1/100. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.
ICC/IF 1/100. (see Abreview)
WB Use at an assay dependent concentration. See Abreview.
IHC-FoFr 1/100. (PubMed:17425404)

靶标

  • 功能

    Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • 组织特异性

    Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • 疾病相关

    Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • 序列相似性

    Belongs to the LAMP family.
  • 翻译后修饰

    O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • 细胞定位

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 数据库链接

  • 形式

    Alternative splicing produces 3 isoforms.
  • 别名

    • CD107 antigen like family member B antibody
    • CD107 antigen-like family member B antibody
    • CD107b antibody
    • LAMP 2 antibody
    • LAMP 2C antibody
    • LAMP-2 antibody
    • LAMP2 antibody
    • LAMP2_HUMAN antibody
    • LAMPB antibody
    • LGP 96 antibody
    • LGP110 antibody
    • LGP96 antibody
    • Lysosomal associated membrane protein 2 antibody
    • Lysosome associated membrane glycoprotein 2 antibody
    • Lysosome associated membrane protein 2 antibody
    • Lysosome-associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane protein 2 antibody
    • MAC3 antibody
    see all

图片

  • Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg

    Secondary
    Goat anti-rat HRP at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 105 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)


    Exposure time: 3 minutes


    Blocked with 5% milk for 1 hour at RT.

    Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.

    See Abreview

  • Immunofluorescence staining of LAMP2 in adult mouse cardiac myocytes using ab13524 at 1/100. Top panels: WT. Lower panels: PINK -/- myocytes. Cells were fixed in 4% PFA and permeabilized with 0.2% saponin. Blocking was performed with 5% normal goat serum + 0.2% Triton X100 in PBS. Hoechst 33342 was used for counter-staining.

  • PFA perfusion-fixed, frozen section of mouse brain tissue stained for LAMP2 using ab13524 at 1/500 dilution in immunohistochemical analysis. Goat anti-rat AlexaFluor® 488 secondary antibody.

    See Abreview

  • ab13524 staining LAMP2 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Rabbit corneal endothelial cell stained for LAMP2 (green) using ab13524 at 1/1000 dilution in ICC/IF.

文献

This product has been referenced in:

  • You JS  et al. The role of raptor in the mechanical load-induced regulation of mTOR signaling, protein synthesis, and skeletal muscle hypertrophy. FASEB J 33:4021-4034 (2019). Read more (PubMed: 30509128) »
  • Iershov A  et al. The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARa. Nat Commun 10:1566 (2019). Read more (PubMed: 30952952) »
See all 84 Publications for this product

客户评价及客户问答

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1-10 of 12 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 18 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 18 2018

Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 16 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% saponin
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jun 27 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Insu Kwon

Verified customer

提交于 Dec 10 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Sep 24 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Aplysia Tissue sections (ganglia)
Specification
ganglia
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

提交于 Dec 09 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Uterus)
Permeabilization
No
Specification
Uterus
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Mukesh Jaiswal

Verified customer

提交于 Nov 29 2013

Application
Western blot
Loading amount
4 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Tissue lysate - whole (uterus)
Specification
uterus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Varkha Agrawal

Verified customer

提交于 Sep 24 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 27 2012

1-10 of 12 Abreviews

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