重组Anti-LAMP1抗体[EPR21026] (ab208943)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21026] to LAMP1
- Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-LAMP1抗体[EPR21026]
参阅全部 LAMP1 一抗 -
描述
兔单克隆抗体[EPR21026] to LAMP1 -
宿主
Rabbit -
特异性
In our lab we observe staining in multiple tissues (spleen, lung, kidney etc.) in IHC-P with ab208943, but a lack of staining on mouse brain. We have received feedback from other researchers, that they also do not see staining in mouse brain with this antibody. Therefore we do not recommend using this reagent, for work on mouse brain, in IHC-P. For further information on this please contact our Technical Support team who will be happy to help.
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经测试应用
适用于: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse lung, colon, kidney and liver lysates; Neuro-2a, RAW 264.7 and NIH/3T3 whole cell lysates. IHC-P: Mouse spleen and lung tissues. IHC-Fr: Mouse kidney tissue. ICC/IF: NIH/3T3 and Neuro-2a cells. Flow Cyt (intra): Neuro-2a cells. IP: NIH/3T3 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21026 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208943于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 43 kDa).
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IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Works well in IHC-P on multiple tissues, but does not work on mouse brain, in this application. |
IHC-Fr |
1/100.
Perform heat mediated antigen retrieval using sodium citrate buffer (pH 6.0). |
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IP |
1/30.
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ICC/IF | (1) |
1/100.
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Flow Cyt (Intra) |
1/500.
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说明 |
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WB
1/1000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 43 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Works well in IHC-P on multiple tissues, but does not work on mouse brain, in this application. |
IHC-Fr
1/100. Perform heat mediated antigen retrieval using sodium citrate buffer (pH 6.0). |
IP
1/30. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/500. |
靶标
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功能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
细胞定位
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 16783 Mouse
- SwissProt: P11438 Mouse
- Unigene: 16716 Mouse
- Unigene: 475822 Mouse
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别名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
图片
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Immunofluorescent analysis of 100% methanol-fixed Neuro-2a (mouse neuroblastoma cell line) cells labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889), at 1/200 dilution (red).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methonol-permeabilized Neuro-2a (mouse neuroblastoma cell line) cell line labeling LAMP1 with ab208943 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-LAMP1 antibody [EPR21026] (ab208943) at 1/2000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-2: 3 minutes; Lane 3: 5 seconds; Lane 4: 10 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
The varying band sizes are due to different levels of glycosylation (PMID: 10212251, PMID: 26246576).
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling LAMP1 with ab208943 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse spleen was observed, performed on a Leica Biosystems BOND® RX instrument (PMID: 22008915). Counter stained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.Note: BOND® is a registered trademark of Leica Biosystems Melbourne Pty Ltd.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line.The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889), at 1/200 dilution (red).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling LAMP1 with ab208943 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse spleen was observed, performed on a Leica Biosystems BOND® RX instrument (PMID: 22008915). Counter stained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.Note: BOND® is a registered trademark of Leica Biosystems Melbourne Pty Ltd.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse kidney tissue labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in the endothelial cells of glomeruli and epithelial cells of renal tubules (PMID: 23229015; PMID:23635510). The nuclear counter stain is DAPI (blue).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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All lanes : Anti-LAMP1 antibody [EPR21026] (ab208943) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse colon lysate
Lane 3 : Mouse liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted?
Exposure time : Lanes 1-2: 15 seconds; Lane 3: 5 seconds.
Blocking and dilution buffer: 5% NFDM/TBST. -
LAMP1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab208943 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208943 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2: ab208943 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208943 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (21)
ab208943 被引用在 21 文献中.
- Guo Z et al. Activity-dependent PI4P synthesis by PI4KIIIα regulates long-term synaptic potentiation. Cell Rep 38:110452 (2022). PubMed: 35235793
- Sun L et al. Failure Of Hearing Acquisition in Mice With Reduced Expression of Connexin 26 Correlates With the Abnormal Phasing of Apoptosis Relative to Autophagy and Defective ATP-Dependent Ca2+ Signaling in Kölliker's Organ. Front Cell Neurosci 16:816079 (2022). PubMed: 35308122
- Liu B et al. Locally generated C3 regulates the clearance of Toxoplasma gondii by IFN-γ-primed macrophage through regulation of xenophagy. Front Microbiol 13:944006 (2022). PubMed: 35992649
- Weng Y et al. Inhibition of the Niemann-Pick C1 protein is a conserved feature of multiple strains of pathogenic mycobacteria. Nat Commun 13:5320 (2022). PubMed: 36085278
- Hsu AP et al. Immunogenetics associated with severe coccidioidomycosis. JCI Insight 7:N/A (2022). PubMed: 36166305