Anti-Laminin抗体[MEC5] (ab2466)

Abreviews (1)Q&A (2)Specific References (2)

概述

  • 产品名称
    Anti-Laminin抗体[MEC5]
    参阅全部 Laminin 一抗
  • 描述
    大鼠单克隆抗体[MEC5] to Laminin
  • 宿主
    Rat
  • 特异性
    This antibody reacts with the P1 fragment of laminin.
  • 经测试应用
    适用于: IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) corresponding to Rat Laminin. ab2466 was derived from DZB rats that had developed membranous glomerulopathy upon exposure to mercuric chloride

性能

应用

Our Abpromise guarantee covers the use of ab2466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/10.

A pepsin pretreatment is required. Especially useful for immunohistochemical detection of laminin in extracellular matrix or basement membranes in freshly frozen tissue sections of rat, mouse or humans.

靶标

文献

This product has been referenced in:
  • Berthod F  et al. Spontaneous fibroblast-derived pericyte recruitment in a human tissue-engineered angiogenesis model in vitro. J Cell Physiol 227:2130-7 (2012). IHC-Fr ; Human . Read more (PubMed: 21769871) »
  • Aten J  et al. Autoantibodies to the laminin P1 fragment in HgCl2-induced membranous glomerulopathy. Am J Pathol 146:1467-80 (1995). Read more (PubMed: 7778685) »

See all 2 Publications for this product

发表研究结果有使用 ab2466？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

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Immunohistochemistry (Frozen sections) abreview for Anti-Laminin antibody [MEC5]

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Sep 08 2009

We are trying to identify sypmathetic efferents in ligaments, so chose a laminin marker to identify smooth muscle associated with blood vessels. We notice that the data sheet for ab2466 says that pepsin pretreatment is required; however, this requireme...

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Thank you for your enquiry regarding ab2466. The antigen retrieval method of pepsin treatment is specific for this antibody as it was found that staining was significantly better with this step. This method is classically used for formalin fixed pa...

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Customer would like to know if the epitopes have been determined for ab2466 and ab6346.

Thank you for your enquiry and for your patience. At this time, all the information that we have regarding these two antibodies is located on the online datasheets. Ab6346: MTn-12 (Aufderheide et al. 1988) reacts specifically with mouse tenascin. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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