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Cardiovascular Angiogenesis Adhesion / ECM Extracellular Matrix

Anti-Laminin抗体[MEC5] (ab2466)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (2)

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Key features and details

  • Rat monoclonal [MEC5] to Laminin
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Laminin抗体[MEC5]
    参阅全部 Laminin 一抗
  • 描述

    大鼠单克隆抗体[MEC5] to Laminin
  • 宿主

    Rat
  • 特异性

    This antibody reacts with the P1 fragment of laminin.
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) corresponding to Rat Laminin. ab2466 was derived from DZB rats that had developed membranous glomerulopathy upon exposure to mercuric chloride

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    MEC5
  • 同种型

    IgG1
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

靶标

  • 功能

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 组织特异性

    Broadly expressed in: skin, heart, lung, and the reproductive tracts.
  • 序列相似性

    Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • 结构域

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • 细胞定位

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Target information above from: UniProt accession Q9Y6N6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10319 Human
    • Entrez Gene: 284217 Human
    • Entrez Gene: 3908 Human
    • Entrez Gene: 3912 Human
    • Entrez Gene: 3913 Human
    • Entrez Gene: 3915 Human
    • Entrez Gene: 3918 Human
    • Entrez Gene: 16772 Mouse
    • Entrez Gene: 316758 Rat
    • Omim: 150320 Human
    • SwissProt: P07942 Human
    • SwissProt: P24043 Human
    • SwissProt: P25391 Human
    • SwissProt: P55268 Human
    • SwissProt: Q9Y6N6 Human
    • SwissProt: P19137 Mouse
    • SwissProt: Q9R0B6 Mouse
    • Unigene: 201805 Human
    • Unigene: 270364 Human
    • Unigene: 302362 Mouse
    see all
  • 别名

    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

实验方案

  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (2)

发表研究结果有使用 ab2466?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2466 被引用在 2 文献中.

  • Berthod F  et al. Spontaneous fibroblast-derived pericyte recruitment in a human tissue-engineered angiogenesis model in vitro. J Cell Physiol 227:2130-7 (2012). IHC-Fr ; Human . PubMed: 21769871
  • Aten J  et al. Autoantibodies to the laminin P1 fragment in HgCl2-induced membranous glomerulopathy. Am J Pathol 146:1467-80 (1995). PubMed: 7778685

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Laminin antibody [MEC5]

Excellent
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (184A1 mammary epithelial)
Permeabilization
Yes - PBS + 0.25% Triton-X
Specification
184A1 mammary epithelial
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Oct 20 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Laminin antibody [MEC5]

Average
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Sep 08 2009

Question

We are trying to identify sypmathetic efferents in ligaments, so chose a laminin marker to identify smooth muscle associated with blood vessels. We notice that the data sheet for ab2466 says that pepsin pretreatment is required; however, this requirement is not mentioned for your other laminin-staining products. Please explain - (we already received our shipment of ab2466 and are now unexpectedly wondering about a pepsin protocol...) thanks a lot -

Read More

Abcam community

Verified customer

Asked on Aug 22 2005

Answer

Thank you for your enquiry regarding ab2466. The antigen retrieval method of pepsin treatment is specific for this antibody as it was found that staining was significantly better with this step. This method is classically used for formalin fixed paraffin embedded sections but in this instance was found to also help the staining procedure on freshly frozen tissue sections of rat,mouse or humans. If using your other laminin antibodies on frozen sections you do not need to pretreat with pepsin unless advised on the datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Read More

Abcam Scientific Support

回复于 Aug 23 2005

Question

Customer would like to know if the epitopes have been determined for ab2466 and ab6346.

Read More

Abcam community

Verified customer

Asked on Jun 07 2004

Answer

Thank you for your enquiry and for your patience. At this time, all the information that we have regarding these two antibodies is located on the online datasheets. Ab6346: MTn-12 (Aufderheide et al. 1988) reacts specifically with mouse tenascin. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of ~210 and ~260 kDa. It also recognizes these tenascin chains and the 230 kDa endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblast lysates under reducing conditions (Aufderheide et al. 1988, Weller et al. 1991). In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I. Ab2466: This antibody reacts with the P1 fragment of laminin. If you have any more questions, please contact us again.

Read More

Abcam Scientific Support

回复于 Jun 17 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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