Anti-Laminin抗体[MEC5] (ab2466)

Reviews (2)Q&A (2)References (2)

Key features and details

  • Rat monoclonal [MEC5] to Laminin
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Laminin抗体[MEC5]
    参阅全部 Laminin 一抗

  • 描述

    大鼠单克隆抗体[MEC5] to Laminin

  • 宿主

    Rat

  • 特异性

    This antibody reacts with the P1 fragment of laminin.

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Full length native protein (purified) corresponding to Rat Laminin. ab2466 was derived from DZB rats that had developed membranous glomerulopathy upon exposure to mercuric chloride

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

靶标

文献 (2)

发表研究结果有使用 ab2466？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2466 被引用在 2 文献中.

  • Berthod F  et al. Spontaneous fibroblast-derived pericyte recruitment in a human tissue-engineered angiogenesis model in vitro. J Cell Physiol 227:2130-7 (2012). IHC-Fr ; Human . PubMed: 21769871
  • Aten J  et al. Autoantibodies to the laminin P1 fragment in HgCl2-induced membranous glomerulopathy. Am J Pathol 146:1467-80 (1995). PubMed: 7778685

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Laminin antibody [MEC5]

 Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (184A1 mammary epithelial)
Permeabilization
Yes - PBS + 0.25% Triton-X
Specification
184A1 mammary epithelial
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Oct 20 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Laminin antibody [MEC5]

 Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Sep 08 2009

Question

We are trying to identify sypmathetic efferents in ligaments, so chose a laminin marker to identify smooth muscle associated with blood vessels. We notice that the data sheet for ab2466 says that pepsin pretreatment is required; however, this requirement is not mentioned for your other laminin-staining products. Please explain - (we already received our shipment of ab2466 and are now unexpectedly wondering about a pepsin protocol...) thanks a lot -

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Answer

Thank you for your enquiry regarding ab2466. The antigen retrieval method of pepsin treatment is specific for this antibody as it was found that staining was significantly better with this step. This method is classically used for formalin fixed paraffin embedded sections but in this instance was found to also help the staining procedure on freshly frozen tissue sections of rat,mouse or humans. If using your other laminin antibodies on frozen sections you do not need to pretreat with pepsin unless advised on the datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Question

Customer would like to know if the epitopes have been determined for ab2466 and ab6346.

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Answer

Thank you for your enquiry and for your patience. At this time, all the information that we have regarding these two antibodies is located on the online datasheets. Ab6346: MTn-12 (Aufderheide et al. 1988) reacts specifically with mouse tenascin. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of ~210 and ~260 kDa. It also recognizes these tenascin chains and the 230 kDa endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblast lysates under reducing conditions (Aufderheide et al. 1988, Weller et al. 1991). In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I. Ab2466: This antibody reacts with the P1 fragment of laminin. If you have any more questions, please contact us again.

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