Full length native protein (purified) corresponding to Human Laminin alpha 5. Database link: O15230
This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab210957 as a replacement.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5 antibody [4C7] (ab17107)This image is courtesy of an anonymous Abreview
ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.
Mackey AL et al. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J25:1943-59 (2011).
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