Anti-Lamin B2抗体(ab97513)
Key features and details
- Rabbit polyclonal to Lamin B2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Lamin B2抗体
参阅全部 Lamin B2 一抗 -
描述
兔多克隆抗体to Lamin B2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 221-470 of Human Lamin B2 (NP_116126)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
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IHC-P |
1/100 - 1/250.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
IHC-P
1/100 - 1/250. |
靶标
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功能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
疾病相关
Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
细胞定位
Nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 84823 Human
- Omim: 150341 Human
- SwissProt: Q03252 Human
- Unigene: 728836 Human
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别名
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
see all
图片
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All lanes : Anti-Lamin B2 antibody (ab97513) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of Lamin B2 in paraffin embedded Cal27 Xenograft, using ab97513 at a 1/100 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab97513 被引用在 3 文献中.
- Ding B et al. Disease Modeling with Human Neurons Reveals LMNB1 Dysregulation Underlying DYT1 Dystonia. J Neurosci 41:2024-2038 (2021). PubMed: 33468570
- Cioni JM et al. Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cell 176:56-72.e15 (2019). PubMed: 30612743
- Yoon BC et al. Local translation of extranuclear lamin B promotes axon maintenance. Cell 148:752-64 (2012). ICC/IF ; Xenopus laevis . PubMed: 22341447