Application
Western blot
Sample
Zebrafish Tissue lysate - whole (retina)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
3e+006 cells
Specification
retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05% PBST
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: sigma Goat@Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 30 kDa
Positive control
did not have any positive control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 08 2022