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Cell Biology Apoptosis Nucleus Lamins
使用敲除细胞株进行验证重组RabMAb

重组Anti-Lamin B1抗体[EPR8985(B)] (ab133741)

  • Datasheet
  • SDS
Reviews (11)Q&A (1)References (93)

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Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
  • Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8985(B)] to Lamin B1
  • Suitable for: IP, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Human LMNB1 (Lamin B1) knockout HeLa cell line (ab255404)

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概述

  • 产品名称

    Anti-Lamin B1抗体[EPR8985(B)]
    参阅全部 Lamin B1 一抗
  • 描述

    兔单克隆抗体[EPR8985(B)] to Lamin B1
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide. within Human Lamin B1 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P20700

  • 阳性对照

    • WB: Hap1, HeLa, Jurkat, Molt4, Y79, Caco 2, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates. Mouse brain,heart, kidney and spleen; and Rat brain, heart and spleen lysates. IHC-P: Human colon, liver and transitional cell carcinoma of the bladder tissues. ICC/IF: Ramos cells, HAP1-LMNB1 cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 解离常数(KD)

    KD = 1.95 x 10 -10 M
    Learn more about KD
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR8985(B)
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Lamins
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins
    • Isotype/Loading Controls
    • Loading Controls
    • Lamin B1 - Nuclear
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Lamins

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    • Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)
    • HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    • Alexa Fluor® 594 Anti-Lamin B1 antibody [EPR8985(B)] (ab216723)
    • Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human LMNB1 (Lamin B1) knockout HeLa cell line (ab255404)
  • KO cell lysates

    • Human LMNB1 (Lamin B1) knockout HeLa cell lysate (ab263825)
  • KO cell pellets

    • Human LMNB1 (Lamin B1) knockout HeLa cell pellet (ab278992)
  • Positive Controls

    • NIH/3T3 nuclear extract lysate (ab14874)
    • PC-12 nuclear extract lysate (ab14884)
    • Y79 nuclear extract lysate (ab14915)
    • NIH/3T3 whole cell lysate (ab7179)
    • MOLT-4 whole cell lysate (ab7912)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Human
IHC-P
Mouse
Human
IP
Human
WB
Mouse
Rat
Human
应用 Ab评论 说明
IP
1/20.
ICC/IF (3)
Use a concentration of 1 µg/ml.
WB (7)
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IHC-P (1)
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

说明
IP
1/20.
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

靶标

  • 功能

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • 疾病相关

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • 序列相似性

    Belongs to the intermediate filament family.
  • 翻译后修饰

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • 细胞定位

    Nucleus inner membrane.
  • Target information above from: UniProt accession P20700 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4001 Human
    • Entrez Gene: 16906 Mouse
    • Entrez Gene: 116685 Rat
    • Omim: 150340 Human
    • SwissProt: P20700 Human
    • SwissProt: P14733 Mouse
    • SwissProt: P70615 Rat
    • Unigene: 89497 Human
    • Unigene: 4105 Mouse
    • Unigene: 11362 Rat
    see all
  • 别名

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

图片

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LMNB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 66-70 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab133741 observed at 66-70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab133741 was shown to react with LMNB1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255404 (knockout cell lysate ab263825) was used. Wild-type HeLa and LMNB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133741 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling Lamin B1 with ab133741 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/200 dilution (green). Nuclear envelope staining on Ramos cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab133741 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/200 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of the bladder tissue labeling Lamin B1 with purified ab133741 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

    ab133741 staining Lamin B1 in wild-type HAP1 cells (top panel) and Lamin B1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab133741 at 1μg/ml dilution and ab195889 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with ab150081 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Lanes 1 & 3 : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/1000 dilution


    Lane 1 : Wild-type HAP1 whole cell lysate at 20 µg
    Lanes 2 & 4 : Empty
    Lane 3 : LMNB1 knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 66 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab133741 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133741 was shown to specifically react with Lamin B1 in wild type HAP1 cells. No band was observed when knockout samples were used. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Ab133741 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Lanes 1 & 3 : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)


    Lane 1 : Wild-type HAP1 whole cell lysate at 20 µg
    Lane 2 : Empty
    Lane 3 : Lamin B1 knockout HAP1 cell lysate at 20 µg

    Predicted band size: 66 kDa



    Lanes 1 - 3: Merged signal (red and green).

    Green - Target observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab133741 and a competitor's discontinued goat polyclonal antibody.

     

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/1000 dilution + GST-tagged Recombinant Human Lamin B1 protein (aa 1 to 586) at 0.015 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Recombinant Human Lamin B1 protein (ab114163)

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/50000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood)
    cell lysate
    Lane 2 : Molt-4 (Human lymphoblastic leukemia cell line) cell lysate
    Lane 3 : Y79 (Human retinoblastoma cell line) cell lysate
    Lane 4 : Caco-2 (Human colorectal adenocarcinoma cells) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

    Immunohistochemical staining of paraffin embedded Mouse Cerebral cortex with purified ab133741 at a working dilution of 1/300. The secondary antibody used is a HRP polymer for rabbit IgG. Nuclear envelope staining on neuron cells of Cerebral cortex tissue is observed. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/50000 dilution (purified)

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 3 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
    Lane 4 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/10000 dilution (purified) + Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Lamin B1 with unpurified ab133741 at 1/250.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/10000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse spleen lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates
    Lane 7 : Rat spleen lysates
    Lane 8 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 10 : NIH/3T3 (Mouse embyro fibroblast cells)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lamin B1 with unpurified ab133741 at 1/250.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

    ab133741 (purified) at 1/20 immunoprecipitating Lamin B1 in Jurkat cells (Lane 1).  For western blotting, ab133741 was used at 1/1000 dilution and an HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)
    Anti-Lamin B1 antibody [EPR8985(B)] (ab133741)

实验方案

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (93)

发表研究结果有使用 ab133741?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab133741 被引用在 93 文献中.

  • Arreal L  et al. Targeting PML in triple negative breast cancer elicits growth suppression and senescence. Cell Death Differ 27:1186-1199 (2020). PubMed: 31570853
  • Oksanen M  et al. NF-E2-related factor 2 activation boosts antioxidant defenses and ameliorates inflammatory and amyloid properties in human Presenilin-1 mutated Alzheimer's disease astrocytes. Glia 68:589-599 (2020). PubMed: 31670864
  • Wang WP  et al. Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis. Aging Cell 19:e13090 (2020). PubMed: 31833196
  • Yu X  et al. Asiatic acid ameliorates obesity-related osteoarthritis by inhibiting myeloid differentiation protein-2. Food Funct 11:5513-5524 (2020). PubMed: 32514515
  • Li W  et al. Lamin B1 Overexpresses in Lung Adenocarcinoma and Promotes Proliferation in Lung Cancer Cells via AKT Pathway. Onco Targets Ther 13:3129-3139 (2020). PubMed: 32346296
View all Publications for this product

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1-10 of 12 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hiPSC)
Permeabilization
Yes - 0.1% Triton X
Specification
hiPSC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Methanol
Read More

Abcam user community

Verified customer

提交于 Jul 08 2019

Western blot abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (ARPE-19 cells)
Gel Running Conditions
Reduced Denaturing (4-12% BisTris Gel)
Loading amount
10 µg
Treatment
H2O2 for 10 min
Specification
ARPE-19 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 May 21 2019

Western blot abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - nuclear (mouse RPE nuclear fractions)
Gel Running Conditions
Reduced Denaturing (4-12% BisTris Gel)
Loading amount
10 µg
Treatment
smoke extract injection
Specification
mouse RPE nuclear fractions
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Lei Wang

Verified customer

提交于 May 21 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cell)
Permeabilization
Yes - 0.1% Triton 100
Specification
Melanoma cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Apr 12 2019

Western blot abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Aug 01 2018

Western blot abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
293T cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 May 03 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Intestine, spleen, liver.)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9
Permeabilization
No
Specification
Intestine, spleen, liver.
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Mr. Victor Bernal

Verified customer

提交于 Apr 23 2018

Western blot abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse macrophage cell line)
Gel Running Conditions
Reduced Denaturing (10% acylamide)
Loading amount
10 µg
Specification
mouse macrophage cell line
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Mar 28 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Kidney)
Permeabilization
Yes - 0.5% triton x 100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Mr. Aravindh Subramani

Verified customer

提交于 Apr 18 2016

Western blot abreview for Anti-Lamin B1 antibody [EPR8985(B)]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cell lines: SH-SY5Y (neuronal, human) MLP29 (hepat)
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Cell lines: SH-SY5Y (neuronal, human) MLP29 (hepat
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.05% · Temperature: RT°C
Read More

Abcam user community

Verified customer

提交于 Jan 28 2016

1-10 of 12 Abreviews or Q&A

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