Anti-Lamin B Receptor/LBR抗体(ab169306)
Key features and details
- Mouse polyclonal to Lamin B Receptor/LBR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Lamin B Receptor/LBR抗体
参阅全部 Lamin B Receptor/LBR 一抗 -
描述
小鼠多克隆抗体to Lamin B Receptor/LBR -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Orangutan -
免疫原
Recombinant full length protein within Human Lamin B Receptor/LBR aa 1-650. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q14739 -
阳性对照
- Lamin B Receptor/LBR transfected 293T cell lysate; Jurkat cell lysate; Human small intestine tissue; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab169306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Anchors the lamina and the heterochromatin to the inner nuclear membrane. -
疾病相关
Defects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis. -
序列相似性
Belongs to the ERG4/ERG24 family. -
翻译后修饰
Phosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. -
细胞定位
Nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3930 Human
- Omim: 600024 Human
- SwissProt: Q14739 Human
- SwissProt: Q5R7H4 Orangutan
- Unigene: 435166 Human
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别名
- DHCR 14B antibody
- DHCR14B antibody
- Integral nuclear envelope inner membrane protein antibody
see all
图片
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Anti-Lamin B Receptor/LBR antibody (ab169306) at 1 µg/ml + Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 71 kDa -
All lanes : Anti-Lamin B Receptor/LBR antibody (ab169306) at 1 µg/ml
Lane 1 : Lamin B Receptor/LBR transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling Lamin B Receptor/LBR with ab169306 at 3µg/ml.
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Immunofluorescent analysis of HeLa cells labeling Lamin B Receptor/LBR with ab169306 at 10µg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab169306 被引用在 3 文献中.
- Wang J et al. 7-Hydroxycoumarin Attenuates Colistin-Induced Kidney Injury in Mice Through the Decreased Level of Histone Deacetylase 1 and the Activation of Nrf2 Signaling Pathway. Front Pharmacol 11:1146 (2020). PubMed: 32848758
- Chen K et al. Protective effect of histone methyltransferase NSD3 on ISO-induced cardiac hypertrophy. FEBS Lett 593:2556-2565 (2019). PubMed: 31254363
- Jeong K et al. Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane. Nucleic Acids Res 43:3114-27 (2015). Rat . PubMed: 25753664