Key features and details
- Mouse monoclonal [3D12] to Lambda Free Light Chain
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
产品名称Anti-Lambda Free Light Chain抗体[3D12]
描述小鼠单克隆抗体[3D12] to Lambda Free Light Chain
特异性Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.
经测试应用适用于: IHC-Pmore details
Mixture of light chains of human immunoglobulin.
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
常规说明Concentration varies from lot to lot and can be provided on request.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Concentration information loading...
纯度Protein A purified
纯化说明Purity tested by electrophoresis.
Our Abpromise guarantee covers the use of ab1944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.