Anti-LAMB3抗体(ab97765)
Key features and details
- Rabbit polyclonal to LAMB3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LAMB3抗体
参阅全部 LAMB3 一抗 -
描述
兔多克隆抗体to LAMB3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human LAMB3 aa 644-930.
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阳性对照
- H1299, HeLa, or HepG2 and cell lines. NIH-3T3 and A431 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97765于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 130 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 130 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
组织特异性
Found in the basement membranes (major component). -
疾病相关
Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
序列相似性
Contains 6 laminin EGF-like domains.
Contains 1 laminin N-terminal domain. -
结构域
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain VI is globular. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3914 Human
- Entrez Gene: 16780 Mouse
- Omim: 150310 Human
- SwissProt: Q13751 Human
- SwissProt: Q61087 Mouse
- Unigene: 497636 Human
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别名
- BM600 125KDA antibody
- Epiligrin subunit bata antibody
- FLJ99565 antibody
see all
图片
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Anti-LAMB3 antibody (ab97765) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 130 kDa
7.5% SDS PAGE -
Anti-LAMB3 antibody (ab97765) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 130 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa, using ab97765 at 1:200.
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Immunohistochemical analysis of paraffin-embedded human lung cancer, using ab97765 to detect LAMB3 at 1:250.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97765 被引用在 1 文献中.
- Curty G et al. Human Endogenous Retrovirus Expression Is Upregulated in the Breast Cancer Microenvironment of HIV Infected Women: A Pilot Study. Front Oncol 10:553983 (2020). PubMed: 33194615