重组Anti-LAGE-1抗体[EPR27371-91] (ab307732)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27371-91] to LAGE-1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-LAGE-1抗体[EPR27371-91]
参阅全部 LAGE-1 一抗 -
描述
兔单克隆抗体[EPR27371-91] to LAGE-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Human testis lysates. IHC-P: Human testis tissues. IP: Human testis cell.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27371-91 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab307732于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 21 kDa.
|
|
IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IP |
1/30.
|
说明 |
---|
WB
1/1000. Predicted molecular weight: 21 kDa. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
靶标
-
组织特异性
Testis and very low level in placenta and in some uterus samples. Observed in 25-50% of tumor samples of melanomas, non-small-cell lung carcinomas, bladder, prostate and head and neck cancers. -
序列相似性
Belongs to the CTAG family. - Information by UniProt
-
数据库链接
- Entrez Gene: 30848 Human
- Omim: 300396 Human
- SwissProt: O75638 Human
- Unigene: 87225 Human
-
别名
- Autoimmunogenic cancer/testis antigen NY ESO 2 antibody
- Autoimmunogenic cancer/testis antigen NY-ESO-2 antibody
- CAMEL antibody
see all
图片
-
All lanes : Anti-LAGE-1 antibody [EPR27371-91] (ab307732) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: human kidney (PMID: 10399963).
Exposure time: 180 seconds
-
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling LAGE-1 with ab307732 at 1/2000 (0.245 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human testis (PMID: 10399963). The section was incubated with ab307732 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LAGE-1 with ab307732 at 1/2000 (0.245 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: no staining on human kidney (PMID: 10399963). The section was incubated with ab307732 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
LAGE-1 was immunoprecipitated from 0.35 mg Human testis tissue lysate 10 ug with ab307732 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307732 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human testis tissue lysate 10 ug
Lane 2: ab307732 IP in Human testis tissue lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab307732 in human testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab307732 尚未被引用在任何文献中。