Anti-Lactate Dehydrogenase抗体(ab231903)
Key features and details
- Rabbit polyclonal to Lactate Dehydrogenase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-Lactate Dehydrogenase抗体
参阅全部 Lactate Dehydrogenase 一抗 -
描述
兔多克隆抗体to Lactate Dehydrogenase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human, Pig
预测可用于: Rat, Rabbit, Cow, Chimpanzee, Macaque monkey, Orangutan -
免疫原
Recombinant full length protein corresponding to Pig Lactate Dehydrogenase aa 1 to the C-terminus. N-terminal His-tag and GST-tag. Expressed in E. coli.
Database link: P00339 -
阳性对照
- WB: Recombinant porcine Lactate Dehydrogenase protein; porcine serum and kidney tissue lysate; mouse testis tissue lysate; A431 and A375 whole cell lysate. IHC-P: Porcine skeletal muscle and heart tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab231903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 37 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 37 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
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通路
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
疾病相关
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
序列相似性
Belongs to the LDH/MDH superfamily. LDH family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 281275 Cow
- Entrez Gene: 3939 Human
- Entrez Gene: 3945 Human
- Entrez Gene: 16828 Mouse
- Entrez Gene: 16832 Mouse
- Entrez Gene: 407246 Pig
- Entrez Gene: 24533 Rat
- Entrez Gene: 24534 Rat
see all -
别名
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase B chain antibody
see all
图片
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Formalin-fixed, paraffin-embedded porcine skeletal muscle tissue stained for Lactate Dehydrogenase using ab231903 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Lactate Dehydrogenase antibody (ab231903) at 3 µg/ml
Lane 1 : Porcine serum
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Lane 4 : Porcine kidney tissue lysate
Lane 5 : Mouse testis tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Formalin-fixed, paraffin-embedded porcine heart tissue stained for Lactate Dehydrogenase using ab231903 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Lactate Dehydrogenase antibody (ab231903) at 3 µg/ml + Recombinant porcine Lactate Dehydrogenase protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab231903 尚未被引用在任何文献中。