• 产品名称
    参阅全部 KRAS+HRAS+NRAS 一抗
  • 描述
    小鼠单克隆抗体to KRAS+HRAS+NRAS
  • 宿主
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment (GST-tag) corresponding to Human KRAS+HRAS+NRAS aa 16-125.


    Database link: P01116

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab206969 as a replacement.



Our Abpromise guarantee covers the use of ab55391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.



  • 相关性
    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Enzyme regulation: Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP.
  • 细胞定位
    Cell Membrane, Cytoplasmic and Golgi Apparatus
  • 数据库链接
  • 别名
    • c-H-ras antibody
    • c-K-ras antibody
    • c-Ki-ras antibody
    • GTPase HRas antibody
    • GTPase KRas antibody
    • GTPase NRas antibody
    • H-Ras-1 antibody
    • Ha-Ras antibody
    • HRAS1 antibody
    • K-Ras 2 antibody
    • Ki-Ras antibody
    • KRAS antibody
    • KRAS2 antibody
    • NRAS antibody
    • p21ras antibody
    • RASK2 antibody
    • Transforming protein N-Ras antibody
    • Transforming protein p21 antibody
    see all


  • cK Ras antibody (ab55391) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab55391 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55391, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

  • ab55391 staining KRAS+HRAS+NRAS in Human Gastric Acenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% phosphate buffered formalin, permeabilized with 0.025% of triton X -100 in TBS and blocked with 3% hydrogen peroxide for 5 minutes at room temperature; antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody (1/100 in 0.025% triton X-100-TBS) for 1 hour. An HRP polymer was used for detection.


This product has been referenced in:
  • Zhang L  et al. Loss of ARID1A Expression Correlates With Tumor Differentiation and Tumor Progression Stage in Pancreatic Ductal Adenocarcinoma. Technol Cancer Res Treat 17:1533034618754475 (2018). Read more (PubMed: 29486633) »
  • Yoo JY  et al. KRAS Activation and over-expression of SIRT1/BCL6 Contributes to the Pathogenesis of Endometriosis and Progesterone Resistance. Sci Rep 7:6765 (2017). Read more (PubMed: 28754906) »

See all 15 Publications for this product


Western blot
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
1e+006 cells
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

提交于 Jan 12 2016

The immunogen for this antibody is KRAS (NP_004976, 16 a.a. ~ 126 a.a). As we don't do epitope mapping, we can't provide you the exactly epitope where the antibody is targeting on. Therefore without testing, we cannot be sure if the mutated form of KRA...

Read More

Thank you for contacting Abcam earlier today.

I am sorry that you were having some issues with ab55391. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples . Based on the protocol info...

Read More

Merci pour ces précisions.

Comme convenu j'ai mis en place l'envoi d'une unité d'anti-KRAS ab55391. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmati...

Read More

Thank you for contacting us.

The current in stock lots are GR47401-1, GR47401-2.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam.

Under our Abpromise we guarantee that all of those antibodies you listed would work on human tissue in IHC-P. However I would recommend ab55391, as It has protocol information available on the website.
Read More

Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab84573.

Notre poli...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibodyhas not been providing satisfactory results despite the protocol tips provided.

The details provided will enable us to investigate...

Read More

Thank you for clarifying the situation. Having reviewed the results and the protocol used I would recommend the following:

1.) Although I have found reference of KRAS expression in some of the used cell lines. I would still suggest usin...

Read More

Thank you for taking time to liaising with the customer and to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. Having reviewed this case, I would like to offer some suggestions to help optimize t...

Read More

1-10 of 15 Abreviews or Q&A