概述

  • 产品名称
  • 描述
    山羊多克隆抗体to KPNB1
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human KPNB1 aa 851-863 (C terminal).
    Sequence:

    RRSKTNKAKTLAT

  • 阳性对照
    • Daudi cell lysate (WB), Human Spleen tissue (IHC-P)
  • 常规说明

    Previously labelled as NTF97/Importin beta. 

性能

应用

Our Abpromise guarantee covers the use of ab115882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • 序列相似性
    Belongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • 细胞定位
    Cytoplasm. Nucleus envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IMB 1 antibody
    • IMB1 antibody
    • IMB1_HUMAN antibody
    • Impnb antibody
    • Importin 90 antibody
    • Importin beta 1 antibody
    • Importin beta 1 subunit antibody
    • Importin subunit beta-1 antibody
    • Importin-90 antibody
    • IPOB antibody
    • Karyopherin beta 1 antibody
    • Karyopherin beta 1 subunit antibody
    • Karyopherin subunit beta-1 antibody
    • KPNB 1 antibody
    • Kpnb1 antibody
    • MGC2155 antibody
    • MGC2156 antibody
    • MGC2157 antibody
    • NTF 97 antibody
    • NTF97 antibody
    • NTF97/Importin beta antibody
    • Nuclear factor P97 antibody
    • Pore targeting complex 97 kDa subunit antibody
    • PTAC97 antibody
    see all

图片

  • Anti-KPNB1 antibody (ab115882) at 0.03 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 97 kDa

  • Human Spleen (formalin-fixed, paraffin-embedded) stained with ab115882 at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

文献

ab115882 has not yet been referenced specifically in any publications.

客户评价及客户问答

Answer

Thank you for contacting us.


I have run alignments of the immunogens used to create each of these products against drosophila Importin beta. Unfortunately, each immunogen had less than 70% homology with your target and most were less than 50%. We cannot recommend a product or offera product for testingif the matched identity of the immunogen is less than 85%.

I hope this information is helpful and wish you luck in finding a Importin beta antibody to suit your needs.Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册