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Signal Transduction Protein Trafficking Nuclear Import / Export

Anti-KPNA4抗体(ab6039)

  • Datasheet
Reviews (3)Q&A (3)References (20)

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Western blot - Anti-KPNA4 antibody (ab6039)

    Key features and details

    • Goat polyclonal to KPNA4
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-KPNA4抗体
      参阅全部 KPNA4 一抗
    • 描述

      山羊多克隆抗体to KPNA4
    • 宿主

      Goat
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Rat, Human
      预测可用于: Cow, Dog
    • 免疫原

      Synthetic peptide corresponding to Human KPNA4 aa 500-600 (C terminal).
      Database link: NP_002259

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • WB: Human ovary lysate. Mouse ovary and testis lysate. Rat testis lysate.
    • 常规说明

      GenBank Accession Number – NP_002259.


      The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 99% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Primary antibody说明

      The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Trafficking
      • Nuclear Import / Export

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Recombinant Human KPNA4 protein (ab114626)
    • Recombinant Protein

      • Recombinant Human KPNA4 protein (ab114626)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6039于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).

    A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB.

    说明
    WB
    Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).

    A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB.

    靶标

    • 功能

      Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
    • 组织特异性

      Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.
    • 序列相似性

      Belongs to the importin alpha family.
      Contains 10 ARM repeats.
      Contains 1 IBB domain.
    • 结构域

      Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
      The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
      The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
    • 细胞定位

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession O00629 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 535090 Cow
      • Entrez Gene: 478680 Dog
      • Entrez Gene: 3840 Human
      • Entrez Gene: 16649 Mouse
      • Entrez Gene: 361959 Rat
      • Omim: 602970 Human
      • SwissProt: O00629 Human
      • SwissProt: O35343 Mouse
      • Unigene: 467866 Human
      • Unigene: 30601 Mouse
      see all
    • 别名

      • FLJ31113 antibody
      • IMA3_HUMAN antibody
      • Importin alpha 3 antibody
      • Importin alpha 4 subunit antibody
      • Importin alpha Q1 antibody
      • Importin subunit alpha 3 antibody
      • Importin subunit alpha-3 antibody
      • IPO A3 antibody
      • IPOA 3 antibody
      • IPOA3 antibody
      • Karyopherin alpha 4 (importin alpha 3) antibody
      • Karyopherin alpha 4 antibody
      • Karyopherin alpha 4 importin alpha 3 antibody
      • Karyopherin alpha 4 subunit antibody
      • Karyopherin subunit alpha 4 antibody
      • Karyopherin subunit alpha-4 antibody
      • KPNA 4 antibody
      • Kpna4 antibody
      • MGC12217 antibody
      • MGC26703 antibody
      • OTTHUMP00000213880 antibody
      • QIP 1 antibody
      • Qip1 antibody
      • Qip1 protein antibody
      • SRP 3 antibody
      • SRP3 antibody
      see all

    图片

    • Western blot - Anti-KPNA4 antibody (ab6039)
      Western blot - Anti-KPNA4 antibody (ab6039)
      Lanes 1-2 : Anti-KPNA4 antibody (ab6039) at 0.1 µg/ml
      Lane 3 : Anti-KPNA4 antibody (ab6039) at 0.3 µg/ml
      Lane 4 : Anti-KPNA4 antibody (ab6039) at 0.03 µg/ml

      Lane 1 : Human ovary lysate
      Lane 2 : Mouse testis lysate
      Lane 3 : Mouse ovary lysate
      Lane 4 : Rat testis lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 58 kDa



      Lysates in RIPA buffer.

      Detected by chemiluminescence.

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (20)

    发表研究结果有使用 ab6039?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6039 被引用在 20 文献中.

    • Liu G  et al. CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia-induced damage. Mol Med Rep 23:N/A (2021). PubMed: 33537819
    • Peruzzo R  et al. Exploiting pyocyanin to treat mitochondrial disease due to respiratory complex III dysfunction. Nat Commun 12:2103 (2021). PubMed: 33833234
    • Xie Q  et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence 12:754-765 (2021). PubMed: 33616472
    • Hu RH  et al. LncRNA ST7-AS1, by regulating miR-181b-5p/KPNA4 axis, promotes the malignancy of lung adenocarcinoma. Cancer Cell Int 20:568 (2020). PubMed: 33327962
    • Witkowski M  et al. Vascular miR-181b controls tissue factor-dependent thrombogenicity and inflammation in type 2 diabetes. Cardiovasc Diabetol 19:20 (2020). PubMed: 32066445
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KPNA4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 11 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KPNA4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreatic tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
    Permeabilization
    No
    Specification
    pancreatic tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 29 2015

    Western blot abreview for Anti-KPNA4 antibody

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116, HT29 and Caco2 colon cancer cell lines)
    Loading amount
    30 µg
    Specification
    HCT116, HT29 and Caco2 colon cancer cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 07 2006

    Question

    Thank you for your advice; we will analyse other human cell lines and will let you know the results.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    I hope that you are well.

    I am sending this email to inquire about the updates you might have about the WB analysis. There were few anti KPNA4 products that were proving difficult to use and as per your last email you were suppose to contact me with the results.

    I am wondering if these products are working now. Could you update me with any progress you have made? If you are still having problems then please let me know I will certainly try to find a solution of problem.

    Many thanks for your cooperation in this case. I will be looking forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 Jul 26 2012

    Question

    Thank you very much for providing the replacement antibodies. We have now tested these in Western blotting and below is a summary of the results.


    Anti-KPNA4 (ab6039)

    This antibody produces a good signal in Western blot. However, this signal is still much weaker than the signal produced by anti-KPNA2 (ab6036) (used as a positive control) (see top panels inattached PowerPoint file). Interestingly, the signal is also weaker than the signal produced by the same antibody purchased fromAbcam in September 2006 (lower panels). Overall, although it might not be ideal, anti-KPNA4 (ab6039) clearly works and could be useful in our project.

    Anti-KPNA6 (ab82149)

    This antibody produces a doublet at the expected position forKPNAisoforms (between 50-75 kDa), suggesting cross-reaction with another KPNA. In addition, it produces multiple bands at higher mol. wt. some of these as strong as the bands at the expected position. Due to the cross-reaction and the overall low signal (compared to the positive control anti-KPNA2 (ab6036)) this antibody is unlikely to be useful for our project.

    Anti-SRP1 (ab6035)

    This antibody produces multiple bands of low intensity at positions higher than expected fromSRP1 (expected to run between 50-75 kDa). Overall, this antibody failed in Western blot and is of no use for our project.

    In summary, we would like to test further antibodies forKPNA6 andSRP1. Could you please recommend any alternatives and provide them as replacements?

    Thank you very much for your help and advice.

    With regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 01 2012

    Answer

    Thank you for your email.

    Two SRP1 antibodies failed, which is very strange. We have used SRP1 transfected 293T cells line in the characterization of antibody ab124646, this means that the 293T cells seems not expressing the protein. I would suggest using Hela cell line lysates as positive control.

    KPAN6: please use 293 cell line lysates, human skeletal muscle lysates or brain lysates as positive control.

    I am sure these antibodies will produce excellent results once correct cell line is used and with some protocol optimizations.

    I hope this information will be helpful. Should you have any question please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    回复于 Jun 01 2012

    Question

    I have discussed your recommended replacement antibodies with. We would like to try the 3 antibodies ab6035, ab6039 and ab82149 you have suggested.Thank you for all your help.

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1092038.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 May 18 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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