Anti-KPNA4抗体(ab6039)
Key features and details
- Goat polyclonal to KPNA4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-KPNA4抗体
参阅全部 KPNA4 一抗 -
描述
山羊多克隆抗体to KPNA4 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog
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免疫原
Synthetic peptide corresponding to Human KPNA4 aa 500-600 (C terminal).
Database link: NP_002259 -
阳性对照
- WB: Recombinant Human KPNA4 protein (ab114626), human and rat testis and ovary, and mouse testis tissue lysates.
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常规说明
GenBank Accession Number – NP_002259.The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).
A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB. |
| 说明 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa). A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB. |
靶标
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功能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. -
组织特异性
Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes. -
序列相似性
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
结构域
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 535090 Cow
- Entrez Gene: 478680 Dog
- Entrez Gene: 3840 Human
- Entrez Gene: 16649 Mouse
- Entrez Gene: 361959 Rat
- Omim: 602970 Human
- SwissProt: O00629 Human
- SwissProt: O35343 Mouse
see all -
别名
- FLJ31113 antibody
- IMA3_HUMAN antibody
- Importin alpha 3 antibody
see all
图片
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Western blot - Anti-KPNA4 antibody (ab6039)All lanes : Anti-KPNA4 antibody (ab6039) at 1 µg/ml
Lane 1 : Human testis tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat testis tissue lysate
Lane 5 : Rat ovary tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa35µg total protein per lane in RIPA buffer. Detected by chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab6039 被引用在 16 文献中.
- Witkowski M et al. Vascular miR-181b controls tissue factor-dependent thrombogenicity and inflammation in type 2 diabetes. Cardiovasc Diabetol 19:20 (2020). PubMed: 32066445
- Thiele S et al. Cellular Importin-a3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection. Cell Rep 31:107549 (2020). PubMed: 32320654
- Guo L et al. Phosphorylation of importin-a1 by CDK1-cyclin B1 controls mitotic spindle assembly. J Cell Sci 132:N/A (2019). PubMed: 31434716
- Zheng Y et al. MicroRNA-24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin-a3 and regulating inflammatory responses. Exp Ther Med 15:338-344 (2018). PubMed: 29250154
- Major AT et al. Development of a pipeline for automated, high-throughput analysis of paraspeckle proteins reveals specific roles for importin a proteins. Sci Rep 7:43323 (2017). PubMed: 28240251