Anti-KPNA4抗体(ab6039)
Key features and details
- Goat polyclonal to KPNA4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-KPNA4抗体
参阅全部 KPNA4 一抗 -
描述
山羊多克隆抗体to KPNA4 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog
-
免疫原
Peptide with sequence NSSANVPTEGFQF, from C Terminus of the protein sequence according to NP_002259
-
阳性对照
- Recombinant Human KPNA4 protein (ab114626) can be used as a positive control in WB. Human testis and ovary lysate. Human testis tissue sections and human small intestine sections.
-
常规说明
GenBank Accession Number – NP_002259.The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab6039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 - 2 µg/ml. | |
| WB | Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa). A 1 hour primary incubation is recommended for this product. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB. |
靶标
-
功能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. -
组织特异性
Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes. -
序列相似性
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
结构域
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 535090 Cow
- Entrez Gene: 478680 Dog
- Entrez Gene: 3840 Human
- Entrez Gene: 16649 Mouse
- Entrez Gene: 361959 Rat
- Omim: 602970 Human
- SwissProt: O00629 Human
- SwissProt: O35343 Mouse
see all -
别名
- FLJ31113 antibody
- IMA3_HUMAN antibody
- Importin alpha 3 antibody
see all
图片
-
Western blot - Anti-KPNA4 antibody (ab6039)All lanes : Anti-KPNA4 antibody (ab6039) at 1 µg/ml
Lane 1 : Human testis
Lane 2 : Human ovary
Lane 3 : Mouse testis
Lane 4 : Rat testis
Lane 5 : Rat ovary
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody (ab6039)Image is courtesy of an anonymous AbReview.Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded murine nephric tissue sections, labelling KPNA4 with ab6039 at a dilution of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed through 10mM citrate buffer at pH 6.0. Blocking was with 5% serum incubated for 1 hour at 21°C. Secondary was a polyclonal Donkey anti-Goat Alexa Fluor® 647 conjugate at 1/200. Counterstain is DAPI against nuclear DNA. The Negative control is the same as above, but in the abscence of the primary ab6039.
-
Western blot - Anti-KPNA4 antibody (ab6039)ab6039 at 0.5µ g/ml staining approximately 55-60kDa band of KPNA4 in Human Testis lysate by Western blot (ECL). ab6039 at 0.5µg/ml staining approximately 55-60kDa band of KPNA4 in Human Testis lysate by Western blot (ECL). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody (ab6039)ab6039 (3µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody (ab6039)ab6039 at 5µg/ml staining KPNA4 in human small intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
数据表及文件
文献 (14)
ab6039 被引用在 14 文献中.
- Guo L et al. Phosphorylation of importin-a1 by CDK1-cyclin B1 controls mitotic spindle assembly. J Cell Sci 132:N/A (2019). PubMed: 31434716
- Zheng Y et al. MicroRNA-24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin-a3 and regulating inflammatory responses. Exp Ther Med 15:338-344 (2018). PubMed: 29250154
- Major AT et al. Development of a pipeline for automated, high-throughput analysis of paraspeckle proteins reveals specific roles for importin a proteins. Sci Rep 7:43323 (2017). PubMed: 28240251
- Audsley MD et al. Nucleocytoplasmic trafficking of Nipah virus W protein involves multiple discrete interactions with the nuclear import and export machinery. Biochem Biophys Res Commun 479:429-433 (2016). IP ; Human . PubMed: 27622322
- De Houwer S et al. The HIV-1 integrase mutant R263A/K264A is 2-fold defective for TRN-SR2 binding and viral nuclear import. J Biol Chem 289:25351-61 (2014). WB ; Human . PubMed: 25063804
