Anti-KPNA3抗体(ab6038)

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Western blot - Anti-KPNA3 antibody (ab6038)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA3 antibody (ab6038)

Key features and details

  • Goat polyclonal to KPNA3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-KPNA3抗体
    参阅全部 KPNA3 一抗

  • 描述

    山羊多克隆抗体to KPNA3

  • 宿主

    Goat

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Dog

  • 免疫原

    Synthetic peptide: DPTANLQTKEFNF, corresponding to C terminal amino acids 509-521 of Human KPNA3.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    GenBank Accession Number – NP_002258.


    The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

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性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Primary antibody说明

    The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).

A 1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.

  • 组织特异性

    Ubiquitous. Highest levels in heart and skeletal muscle.

  • 序列相似性

    Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.

  • 结构域

    Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • SRP1 gamma antibody
      • hSRP1 antibody
      • IMA4_HUMAN antibody
      • Importin alpha 3 antibody
      • Importin alpha 3 subunit antibody
      • Importin alpha 4 antibody
      • Importin alpha Q2 antibody
      • Importin subunit alpha-4 antibody
      • IPOA4 antibody
      • Karyopherin alpha 3 antibody
      • Karyopherin alpha 3 importin alpha 4 antibody
      • Karyopherin alpha 3 subunit antibody
      • Karyopherin subunit alpha 3 antibody
      • Karyopherin subunit alpha-3 antibody
      • KPNA 3 antibody
      • Kpna3 antibody
      • Qip2 antibody
      • SRP1 antibody
      • SRP1-gamma antibody
      • SRP1gamma antibody
      • SRP4 antibody
      see all

    图片

    • Western blot - Anti-KPNA3 antibody (ab6038)
      Western blot - Anti-KPNA3 antibody (ab6038)
      ab6038 at 0.1µg/ml staining approximately 55-60kDa band of KPNA3 in Human Testis Lysate by Western blot (ECL). ab6038 at 0.1µg/ml staining approximately 55-60kDa band of KPNA3 in Human Testis Lysate by Western blot (ECL).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA3 antibody (ab6038)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA3 antibody (ab6038)

      ab6038 at 5ug/ml staining KPNA3 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

    文献 (17)

    发表研究结果有使用 ab6038？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6038 被引用在 17 文献中.

    • Gao R  et al. The selective NLRP3-inflammasome inhibitor MCC950 reduces myocardial fibrosis and improves cardiac remodeling in a mouse model of myocardial infarction. Int Immunopharmacol 74:105575 (2019). PubMed: 31299609
    • Lyst MJ  et al. Affinity for DNA Contributes to NLS Independent Nuclear Localization of MeCP2. Cell Rep 24:2213-2220 (2018). PubMed: 30157418
    • Huang S  et al. Pellet coculture of osteoarthritic chondrocytes and infrapatellar fat pad-derived mesenchymal stem cells with chitosan/hyaluronic acid nanoparticles promotes chondrogenic differentiation. Stem Cell Res Ther 8:264 (2017). PubMed: 29141683
    • Baker SA  et al. Karyopherin a 3 and karyopherin a 4 proteins mediate the nuclear import of methyl-CpG binding protein 2. J Biol Chem 290:22485-93 (2015). PubMed: 26245896
    • Carter DM  et al. Proteomic identification of nuclear processes manipulated by cytomegalovirus early during infection. Proteomics 15:1995-2005 (2015). PubMed: 25758553
    View all Publications for this product

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