为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Tags & Cell Markers Subcellular Markers Nucleus Nuclear Pore

Anti-KPNA3抗体(ab6038)

  • Datasheet
Submit a review Submit a question References (19)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-KPNA3 antibody (ab6038)

    Key features and details

    • Goat polyclonal to KPNA3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    你可能会感兴趣

    二抗
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    一抗
    Product image
    Anti-KPNA4 antibody (ab6039)

    查看更多相关产品

    概述

    • 产品名称

      Anti-KPNA3抗体
      参阅全部 KPNA3 一抗
    • 描述

      山羊多克隆抗体to KPNA3
    • 宿主

      Goat
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Dog
    • 免疫原

      Synthetic peptide: DPTANLQTKEFNF, corresponding to C terminal amino acids 509-521 of Human KPNA3.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • WB: Human testis whole cell lysate.
    • 常规说明

      GenBank Accession Number – NP_002258.


      The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Primary antibody说明

      The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Tags & Cell Markers
      • Subcellular Markers
      • Nucleus
      • Nuclear Pore
      • Signal Transduction
      • Protein Trafficking
      • Nuclear Import / Export

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6038于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).

    A 1 hour primary incubation is recommended for this product.

    说明
    WB
    Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).

    A 1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
    • 组织特异性

      Ubiquitous. Highest levels in heart and skeletal muscle.
    • 序列相似性

      Belongs to the importin alpha family.
      Contains 10 ARM repeats.
      Contains 1 IBB domain.
    • 结构域

      Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
      The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
      The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
    • 细胞定位

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession O00505 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3839 Human
      • Omim: 601892 Human
      • SwissProt: O00505 Human
      • Unigene: 527919 Human
      • 别名

        • SRP1 gamma antibody
        • hSRP1 antibody
        • IMA4_HUMAN antibody
        • Importin alpha 3 antibody
        • Importin alpha 3 subunit antibody
        • Importin alpha 4 antibody
        • Importin alpha Q2 antibody
        • Importin subunit alpha-4 antibody
        • IPOA4 antibody
        • Karyopherin alpha 3 antibody
        • Karyopherin alpha 3 importin alpha 4 antibody
        • Karyopherin alpha 3 subunit antibody
        • Karyopherin subunit alpha 3 antibody
        • Karyopherin subunit alpha-3 antibody
        • KPNA 3 antibody
        • Kpna3 antibody
        • Qip2 antibody
        • SRP1 antibody
        • SRP1-gamma antibody
        • SRP1gamma antibody
        • SRP4 antibody
        see all

      图片

      • Western blot - Anti-KPNA3 antibody (ab6038)
        Western blot - Anti-KPNA3 antibody (ab6038)
        ab6038 at 0.1µg/ml staining approximately 55-60kDa band of KPNA3 in Human Testis Lysate by Western blot (ECL). ab6038 at 0.1µg/ml staining approximately 55-60kDa band of KPNA3 in Human Testis Lysate by Western blot (ECL).

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (19)

      发表研究结果有使用 ab6038?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab6038 被引用在 19 文献中.

      • Xie Q  et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence 12:754-765 (2021). PubMed: 33616472
      • Thiele S  et al. Cellular Importin-a3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection. Cell Rep 31:107549 (2020). PubMed: 32320654
      • Gao R  et al. The selective NLRP3-inflammasome inhibitor MCC950 reduces myocardial fibrosis and improves cardiac remodeling in a mouse model of myocardial infarction. Int Immunopharmacol 74:105575 (2019). PubMed: 31299609
      • Lyst MJ  et al. Affinity for DNA Contributes to NLS Independent Nuclear Localization of MeCP2. Cell Rep 24:2213-2220 (2018). PubMed: 30157418
      • Huang S  et al. Pellet coculture of osteoarthritic chondrocytes and infrapatellar fat pad-derived mesenchymal stem cells with chitosan/hyaluronic acid nanoparticles promotes chondrogenic differentiation. Stem Cell Res Ther 8:264 (2017). PubMed: 29141683
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      There are currently no Customer reviews or Questions for ab6038.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      沪ICP备14046373号-1

      沪公网安备 31011502007413号

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2022 艾博抗(上海)贸易有限公司版权所有