Anti-KPNA2抗体(ab6036)

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Key features and details

  • Goat polyclonal to KPNA2
  • Suitable for: IHC-P, ELISA, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-KPNA2抗体
    参阅全部 KPNA2 一抗

  • 描述

    山羊多克隆抗体to KPNA2

  • 宿主

    Goat

  • 经测试应用

    适用于: IHC-P, ELISA, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human KPNA2 aa 518-529 (C terminal).
    Sequence:

    QVQDGAPGTFNF


    Database link: P52292

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: HeLa Lysate; IHC-P: Human testis tissue.

  • 常规说明

    GenBank Accession Number – NP_002257.


    The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Primary antibody说明

    The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/32000.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).

A 1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

  • 组织特异性

    Expressed ubiquitously.

  • 序列相似性

    Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.

  • 结构域

    Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • IMA1_HUMAN antibody
    • Importin alpha 1 antibody
    • Importin alpha 2 antibody
    • Importin alpha 2 subunit antibody
    • Importin alpha P1 antibody
    • Importin subunit alpha-1 antibody
    • IPO A1 antibody
    • IPOA 1 antibody
    • IPOA1 antibody
    • Karyopherin alpha 2 antibody
    • Karyopherin alpha 2 RAG cohort 1 antibody
    • Karyopherin alpha 2 subunit antibody
    • Karyopherin subunit alpha-2 antibody
    • KPNA2 antibody
    • KPNA2 protein antibody
    • Pendulin antibody
    • QIP 2 antibody
    • QIP2 antibody
    • RAG cohort 1 antibody
    • RAG cohort protein 1 antibody
    • RCH 1 antibody
    • RCH1 antibody
    • SRP 1 antibody
    • SRP1 alpha antibody
    • SRP1 antibody
    • SRP1-alpha antibody
    • SRP1alpha antibody
    see all

图片

  • Western blot - Anti-KPNA2 antibody (ab6036)
    Western blot - Anti-KPNA2 antibody (ab6036)
    Anti-KPNA2 antibody (ab6036) at 0.03 µg/ml + HeLa lysate (RIPA buffer) at 35 µg

    Predicted band size: 58 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Primary incubation 1 hour. Detected by chemiluminescence.

文献 (1)

发表研究结果有使用 ab6036？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6036 被引用在 1 文献中.

  • Zhang W  et al. RNA-binding protein HuR regulates nuclear import of protein. J Cell Sci 129:4025-4033 (2016). PubMed: 27609837

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