Anti-KMT6 / EZH2抗体(ab186006)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-KMT6 / EZH2抗体
参阅全部 KMT6 / EZH2 一抗 -
描述
兔多克隆抗体to KMT6 / EZH2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis -
免疫原
Synthetic peptide within Human KMT6/ EZH2 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. CAA64955.1
Database link: Q15910 -
阳性对照
- 293T, HeLa, Jurkat, and Mouse TCMK-1 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab186006于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/500 - 1/2500. Predicted molecular weight: 85 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P | (1) |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/2500. Predicted molecular weight: 85 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. -
组织特异性
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. -
序列相似性
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
发展阶段
Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. -
翻译后修饰
Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 509106 Cow
- Entrez Gene: 475511 Dog
- Entrez Gene: 2146 Human
- Entrez Gene: 14056 Mouse
- Entrez Gene: 100625497 Pig
- Entrez Gene: 100345157 Rabbit
- Entrez Gene: 709075 Rhesus monkey
- Entrez Gene: 100381148 Xenopus laevis
see all -
别名
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
see all
图片
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Lane 1 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg
Lanes 2-4 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 cells.lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 3 minutes -
Mouse plasmacytoma tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Human ovarian tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Detection of KMT6 / EZH2 by Western Blot of Immunprecipitate.
Lane 1: ab186006 at 1 µg/ml labeling KMT6 / EZH2 in 293T whole cell lysate immunoprecipitated using ab186006 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (70)
ab186006 被引用在 70 文献中.
- Niwa Y et al. Cyclin D1 Binding Protein 1 Responds to DNA Damage through the ATM-CHK2 Pathway. J Clin Med 11:N/A (2022). PubMed: 35160302
- Yoon JH et al. lncRNAs UC.145 and PRKG1-AS1 Determine the Functional Output of DKK1 in Regulating the Wnt Signaling Pathway in Gastric Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35625973
- Xiang F & Xu X CirRNA F-circEA-2a Suppresses the Role of miR-3613-3p in Colorectal Cancer by Direct Sponging and Predicts Poor Survival. Cancer Manag Res 14:1825-1833 (2022). PubMed: 35652063
- Chen T et al. LncRNA Airn maintains LSEC differentiation to alleviate liver fibrosis via the KLF2-eNOS-sGC pathway. BMC Med 20:335 (2022). PubMed: 36171606
- Chen YN et al. EZH2 is a potential prognostic predictor of glioma. J Cell Mol Med 25:925-936 (2021). PubMed: 33277782