重组Anti-KMT1B / SUV39H2抗体[EPR18495] (ab190870)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18495] to KMT1B / SUV39H2
- Suitable for: IHC-P, IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-KMT1B / SUV39H2抗体[EPR18495]
参阅全部 KMT1B / SUV39H2 一抗 -
描述
兔单克隆抗体[EPR18495] to KMT1B / SUV39H2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK-293, HeLa, NIH/3T3, SH-SY5Y and MOLT-4 cell lysates; human fetal kidney lysate; human, mouse and rat testis lysates. IHC-P: Human, mouse and rat testis tissues. IP: SH-SY5Y whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18495 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab190870于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/50.
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WB |
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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说明 |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/50. |
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
靶标
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功能
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis. -
序列相似性
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 chromo domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
结构域
Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. -
细胞定位
Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin. - Information by UniProt
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数据库链接
- Entrez Gene: 79723 Human
- Entrez Gene: 64707 Mouse
- Entrez Gene: 364785 Rat
- Omim: 606503 Human
- SwissProt: Q9H5I1 Human
- SwissProt: Q9EQQ0 Mouse
- Unigene: 554883 Human
- Unigene: 128273 Mouse
see all -
别名
- FLJ23414 antibody
- H3 K9 HMTase 2 antibody
- H3-K9-HMTase 2 antibody
see all
图片
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All lanes : Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution
Lane 1 : Wild-type U2-OS whole cell lysate
Lane 2 : SUV39H2 knockout U2-OS whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDaLanes 1 - 4: Merged signal (red and green). Green - ab190870 observed at 47 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab190870 was shown to recognize SUV39H2 in wild-type U2-OS cells as signal was lost at the expected MW in SUV39H2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SUV39H2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab190870 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate. at 20 µg
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
Lane 3 : Human fetal kidney at 10 µg
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 3 : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/10000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47,54 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The full length SUV39H2 orthologues differ in size: human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).
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All lanes : Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/5000 dilution
Lane 1 : Human testis lysate
Lane 2 : Mouse testis lysate
Lane 3 : Rat testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47,54 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The full length SUV39H2 orthologues differ in size: human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).
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Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on germ cells of human testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleuarstaining on germ cells of mouse testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on germ cells of rat testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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KMT1B / SUV39H2 was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab190870 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab190870 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: SH-SY5Y whole cell lysate 10µg (Input).
Lane 2: ab190870 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190870 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab190870 被引用在 6 文献中.
- Yang S et al. SUV39H1 regulates corneal epithelial wound healing via H3K9me3-mediated repression of p27. Eye Vis (Lond) 9:4 (2022). PubMed: 35101125
- Feng S et al. RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity. Nat Commun 13:957 (2022). PubMed: 35177609
- Miao Y et al. Histone methyltransferase SUV39H2 regulates LSD1-dependent CDH1 expression and promotes epithelial mesenchymal transition of osteosarcoma. Cancer Cell Int 21:2 (2021). PubMed: 33397384
- Jing J et al. The histone methyltransferase Suv39h regulates 3T3-L1 adipogenesis. Adipocyte 9:401-414 (2020). PubMed: 32698678
- Grigore F et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases. Neoplasia 22:376-389 (2020). PubMed: 32629178
- Shuai W et al. SUV39H2 promotes colorectal cancer proliferation and metastasis via tri-methylation of the SLIT1 promoter. Cancer Lett 422:56-69 (2018). WB, IHC . PubMed: 29458143