Anti-KLF5抗体(ab137676)
Key features and details
- Rabbit polyclonal to KLF5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-KLF5抗体
参阅全部 KLF5 一抗 -
描述
兔多克隆抗体to KLF5 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Pig -
免疫原
Recombinant fragment corresponding to Human KLF5 aa 69-346.
Database link: Q13887 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137676于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/1000.
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
|
ICC/IF | (1) |
1/100 - 1/1000.
|
说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. -
组织特异性
Expressed only in testis and placenta. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
翻译后修饰
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 535702 Cow
- Entrez Gene: 688 Human
- Entrez Gene: 12224 Mouse
- Entrez Gene: 100038005 Pig
- Omim: 602903 Human
- SwissProt: Q13887 Human
- SwissProt: Q9Z0Z7 Mouse
- Unigene: 508234 Human
see all -
形式
KLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation. -
别名
- Basic transcription element binding protein 2 antibody
- Basic transcription element-binding protein 2 antibody
- BTE binding protein 2 antibody
see all
图片
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All lanes : Anti-KLF5 antibody (ab137676) at 1/5000 dilution
Lane 1 : Non-transfected 293T whole cell extract
Lane 2 : Transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa -
Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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All lanes : Anti-KLF5 antibody (ab137676) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa -
HeLa cells stained for KLF5 (green) using ab137676 at 1/500 dilution in ICC/IF.
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ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500
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Anti-KLF5 antibody (ab137676) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 51 kDa
10% SDS PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (37)
ab137676 被引用在 37 文献中.
- Lu X et al. Testis developmental related gene 1 promotes non-small-cell lung cancer through the microRNA-214-5p/Krüppel-like factor 5 axis. Bioengineered 13:603-616 (2022). PubMed: 34856848
- Han D et al. A balanced Oct4 interactome is crucial for maintaining pluripotency. Sci Adv 8:eabe4375 (2022). PubMed: 35171666
- Mooz J et al. ARAF suppresses ERBB3 expression and metastasis in a subset of lung cancers. Sci Adv 8:eabk1538 (2022). PubMed: 35302851
- Luo A et al. KLF5-induced miR-487a augments the progression of osteosarcoma cells by targeting NKX3-1 in vitro. Oncol Lett 24:258 (2022). PubMed: 35765275
- Wang S et al. LINC00665 affects the malignant biological behavior of ovarian cancer via the miR-148b-3p/KLF5. Syst Biol Reprod Med 68:370-383 (2022). PubMed: 36016468