Anti-KLC2抗体(ab95881)
Key features and details
- Rabbit polyclonal to KLC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KLC2抗体
参阅全部 KLC2 一抗 -
描述
兔多克隆抗体to KLC2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between amino acids 150-200 of Human KLC2 (NP_001128247.1).
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阳性对照
- HeLa cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified using an epitope specific to KLC2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 69 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. -
序列相似性
Belongs to the kinesin light chain family.
Contains 6 TPR repeats. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 64837 Human
- Omim: 611729 Human
- SwissProt: Q9H0B6 Human
- Unigene: 280792 Human
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别名
- Kinesin light chain 2 antibody
- KLC 2 antibody
- KLC2 antibody
- KLC2_HUMAN antibody
图片
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All lanes : Anti-KLC2 antibody (ab95881) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 minutes -
ab95881 at 0.4 µg/ml detecting KLC2 in HeLa whole cell lysate by WB following IP.
Lane 1: KLC2 with 3 µg/mg of lysate
Lane 2: IP with an antibody which recognizes a downstream epitope of KLC2
Lane 3: control IgG
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab95881 被引用在 2 文献中.
- Guardia CM et al. RUSC2 and WDR47 oppositely regulate kinesin-1-dependent distribution of ATG9A to the cell periphery. Mol Biol Cell 32:ar25 (2021). PubMed: 34432492
- Carpentier DC et al. Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex. PLoS Pathog 11:e1004723 (2015). WB, IF . PubMed: 25760349