Mitochondrial assays

Simple assays and biochemicals for your mitochondria and metabolism research

Review our assays for mitochondrial research:

For more detailed information about the different methods used to analyze mitochondria, see our mitochondrial toxicity application guide.

Mitochondrial biogenesis

Measurement of mitochondrial biogenesis is becoming a standard part of drug safety characterization. Our assays measure biogenesis by measuring the ratio of mitochondrial and nuclear-encoded proteins.

Assay

Assay kits

In-Cell ELISA with fluorescence, colorimetric or fluorometric readout

Western blot antibody cocktail

ICC antibody cocktail

Flow cytometry antibody cocktail

ab110217, ab110216, ab140359


​ab123545

ab170194

ab168540

 “I am using this kit [ab110217] for high throughput screening of more than 500 compounds. The kit is highly reproducible and I did not observe any lot to lot variation. I would highly recommend this kit.”

– Dr Analeeb Sajid

Cox1 SDHA v1

Mitochondrial viability

The mitochondrial membrane potential is a key indicator of mitochondria activity levels. A number of fluorescent dyes are available that accumulate in mitochondria due to the membrane potential.

Assay

Readout

Notes

Assay kits

TMRE/ TMRM

Plate reader, microscope, flow cytometer

Most popular Abcam mitochondrial membrane dye assay.
Ex/Em 549/575 nm. Washed out after fixation.

ab113852

JC-1/ JC-10

JC-1 (Ex/Em 530/530–570) and JC-10 (Ex/Em 590/520–570) form red aggregates at high concentrations (unaggregated dye is green). Loss of membrane potential causes loss of dye and increased green fluorescence.

Washed out after fixation.

JC-1:
ab113850

JC-10
(more soluble than JC-1):
ab112134
ab112133

MitoNIR

Plate reader, flow cytometer

Ex/Em 635/660.

ab112149 ab11250

Mito Orange

Ex/Em 540/590.

ab138898 ab138899

JC1 web v1

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Mitchondrial toxicity

Your complete toolkit for investigating mitochondrial toxicity in vitro.

Every year, dozens of pharmaceutical compounds are withdrawn from the market due to adverse effects not discovered during the preclinical or clinical testing. Many of these adverse effects are subtle and do not lead to visible histopathology, but often lead to mitochondrial dysfunction. Therefore, screening for mitochondrial toxicity in the early development stages of a drug is essential.
• Don't know where to start? Our step-by-step guide will help you to find the right assay for you.
• Download a copy of our Mitochondrial toxicity Application Guide
• Looking for the right assay? This guide will help you choose the key parameters of mitochondrial toxicity.
• Download a copy of our Tools for Detecting Mitochondrial Toxicity

OXPHOS enzyme complex activity assays

To investigate the in vitro activity of individual oxidative phosphorylation (OXPHOS) enzyme complexes, we use immunocapture to enable specific biochemical assays for each complex.


Immunocapture activity assay

Complex immunocapture

Toxicity assay

Notes

Capture complex and assay activity

Enable user to develop own assays based on captured complex

Immunocapture purified beef mitochondria for in vitro treatment and assays

Complex I

ab109721

ab109711

ab109903

Complex II

ab109908

ab109799

ab109904

Complex III


ab109800

ab109905 (II + III)

Complex IV

ab109911, ab109909, ab109910

ab109801, ab109860

ab109906

Complex V



ab109907

Complete panel



ab110419

rhoa web v2

OXPHOS protein expression analysis

We have developed antibody cocktails for use in western blotting to enable analysis of all 5 oxphos protein complexes in a single experiment.

Assay

Assay kits

Complex I-V antibody cocktail

ab110413, ab110411, ab110412

Complex II antibody cocktail

ab110410

​Apoptosis

Mitochondria are closely involved in apoptosis and cell death. For an in depth look at apoptosis see our Apoptosis Application Guide or to review available assays, tools and reagents, see our Cell Health Assays Guide.



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