Immunohistochemistry kits and reagents
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Get sharp and strong staining in your immunohistochemistry (IHC) with optimized reagents
Quality reagents are key to the accurate localization and identification of antigens on histological sections. We've learnt this from the thousands of hours that we've spent validating antibodies in IHC.
High sensitivity and consistent results
Find the IHC kits and reagents that you need to get clean, specific staining even when detecting low abundance markers. Alternatively, consult our IHC application guide or view our IHC protocols.
Antigen retrieval
Use optimized buffers for heat-induced antigen retrieval and reagents for enzymatic antigen retrieval
Blocking
Block
- non-specific antibody binding with blocking serums for IHC or use a convenient protein blocking buffer.
- endogenous peroxidases, if using HRP for detection, with hydrogen peroxide blocking reagent.
- endogenous biotin, if using biotin-based amplification, with a biotin blocking agent
Chromogenic signal amplification and visualization
Visualize primary antibody staining using a modern micro-polymer method, and a complete IHC detection micro-polymer kit. Or select what you need from HRP-polymer-conjugated secondary antibodies and chromogenic substrate kits.
Alternatively, use the more traditional streptavidin-biotin (LSAB ABC) method and a complete IHC detection ABC kit. Or select what you need from biotin-conjugated secondary antibodies, streptavidin-enzyme conjugates and chromogenic substrate kits.
If sensitivity is not an issue, then use an HRP conjugated secondary antibody, or a rapid HRP or AP antibody conjugation kit.
Fluorescent signal amplification and visualization
Use a dye-conjugated primary antibody, an Alexa-fluor® dye-conjugated secondary antibody or a rapid antibody-dye conjugation kit.
Mouse antibodies on mouse tissue and multi-color chromogenic IHC
Stop high background caused by anti-mouse secondary antibodies binding to mouse tissues with a mouse-on-mouse kit.
Detect multiple antibodies at the same time with multicolor chromogenic IHC kits.
Counterstaining
Identify the location of antibody staining with chromogenic and fluorescent counterstains and special stains for specific cell types, proteins, carbohydrates and metabolites.
Mounting
Use aqueous mounting media for chromogenic IHC, or specialist mounting media for fluorescent IHC.