Firefly Multiplex Immunoassay Validation Data Graph

Analytes and panels for FirePlex immunoassays

Pre-designed and customized panels are ideal for biomarker discovery projects to clinical validation studies. 

  • Human analytes and panels​

Comprehensively profile up to 70 biomarker candidates simultaneously, directly from human plasma (EDTA, heparin, citrate), serum, urine, saliva, milk (defatted), synovial fluid, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), or cell culture supernatant. FirePlex human immunoassays give you the flexibility, speed, and high-performance to take biomarker studies from the bench to the bedside.

Choose from our pre-designed focus panels below.

Alternatively, design your own custom mouse or human panel request with our quick and easy FirePlex immunoassay panel builder.

Human focus panels 

Panel Name


Analyte List

Fireplex Focused Cytokines


IL-6, IL-10, IFN-γ, TNF-α, Neg. Ctl.

FirePlex Key Cytokines​​


IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-17A, GM-CSF, IFN-γ, MCP1, MIP1-α, MIP1-β, TNF-α, Neg. Ctl.

FirePlex Th1/Th2​​


IL-2, IL-4, IL-5, IL-6, IL-10, IFN-γ, TNF-α

FirePlex Th1/Th2/Th17​​


IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF-α

FirePlex Inflammation​​


IL-1β, IL-6, IL-8, IL-10, IL-12p70, IFN-γ, MCP1, TNF-α

New mid- and large-plex discovery panels offer 36 and 70 analyte compilations for more extensive biomarker analysis and in-depth studies.

Panel NameabIDAnalyte List

FirePlex Discovery Panel 1FirePlex Discovery Panel 1


FirePlex Discovery Panel 2+3 (excluding analytes with *)

FirePlex Discovery Panel 2


AGR2, BCA1, BST2, CD270, CXCL5, Fractalkine, G-CSF, GM-CSF, IFNγ, IL-1β, IL-1Ra, IL-2, IL-4, IL-5, IL-6, IL-6 Rα, IL-8, IL-9, IL-10, IL-12p70, IL-13*, IL-16, IL-17A, IL-23, IP-10, Liver-FABP, MCP1, MDC, Mesothelin, MIP1a, MIP1b, Myoglobin, PCSK9, TARC, TNFα, VEGF R2

FirePlex Discovery Panel 3


Agrin, Axl*, BDNF, Calbindin, CD276, CD40, Coronin 1a, EGF, EMMPRIN, Eotaxin-2, FGF1, FGFR3, GH, Hemoglobin subunit α, ICAM2, IL-1 RI, IL-3, IL-15, IL-18, IL-22, IL-31, IFNα 1, IFNα2, LOX1, MCP2, MIG, MIP2b, MIP5, Oncostatin M, Pentraxin 3, Procalcitonin, sTNF RI, Tissue factor, TREM2, Urokinase type Plasminogen Activator, VEGF-A

Rodent analytes and panels 

FirePlex mouse and rat immunoassay product lines provide essential tools for pre-clinical drug development research, whether identifying disease biomarkers, validating in vitro studies, or screening drug candidates for toxicity.

Offering multiplex analysis of up to 75 analytes per well, directly from plasma (EDTA, heparin, citrate), serum, urine or cell culture supernatant, our specialized mouse panels and analytes offer fast, easy and high-performance biomarker analysis to streamline your research pipeline.

As with our human panels, you can choose from our pre-designed focus panels below and add analytes to create your custom panel suited to your specific research needs:

Mouse focus panels

Panel NameabIDAnalyte List
Focused Cytokines ab235660IFNγ, IL-6, IL-10, TNFα
Key Cytokines ab235656CXCL1, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, MCP1, MIP1α, MIP1β, TNFα
Th1/Th2 ab235657
IL-2, IL-4, IL-5, IL-6, IL-10, TNFα, IFNγ
Th1/Th2/Th17 ab235658IL-2, IL-4, IL-6, IL-10, IL-17A, IFNγ, TNFα
Inflammation ab235659CXCL1, IFNγ, IL-1β, IL-6, IL-10, IL-12p70, MCP1, TNFα
Discovery Panel ab239456BCA1 , CCL27, CD137, CD23 , CD31, CD40, CXCL1, CXCL11, CXCL16, DLL1, Eotaxin, Eotaxin-2, FAS Ligand, Fractalkine, Galectin 3, G-CSF, GM-CSF, GRO γ, HGF, ICAM2, IFNα2, IFN-γ, IL-1a, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-6 Rα, IL-7, IL-9, IL-10, IL-11 , IL-12/23p40, IL-12p70, IL-13, IL-16, IL-17A, IL-17F, IL-18 , IL-19, IL-20, IL-21, IL-22, IL-27a, IL-28, IL-31, IL-33, IP-10, KIM-1, Leptin, MCP1, MCP3, M-CSF, MER, MIF, MIG, MIP1a, MIP1b, MMP8 , Osteopontin, PD1, RAGE, Stem Cell Factor, sTNF RI, TACI, TNFα, VEGFA

Rat focus panels

Panel NameabIDAnalyte List
Key Cytokinesab235662CXCL1, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, MCP1, MIP1α, MIP1β, TNFα
Focused Cytokinesab235666IFNγ, IL-6, IL-10, TNFα
Th1/Th2ab235663IL-2, IL-4, IL-5, IL-6, IL-10, IFNγ, TNFa
Th1/Th2/Th17ab235664IL-2, IL-4, IL-6, IL-10, IL-17A, IFNγ, TNFα
Inflammationab235665CXCL1, IFNγ, IL-1β, IL-6, IL-10, IL-12p70, MCP1, TNFα


Add specific analytes to a panel or design your custom panel from scratch for multiplex analysis of up to 70 analytes simultaneously per well for human or 75 analytes simultaneously per well for rodent. Select from our extensive catalog of hundreds of rodent analytes, including important growth factors, signaling peptides/proteins, and other diverse biomarker candidates.

For product codes, limits of detection, and dynamic range, click on any analyte above for the product datasheet or download our PDF listing. Alternatively, contact us at to find the analyte you need.

*In rare cases, specific analyte combinations may be incompatible, in which case you will be notified before placing your order

Request a free consultation and discuss your specific research needs with our team of profiling experts at there’s anything else you need, please get in touch.

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