为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Firefly particles | miRNA

FirePlex® particle technology

Related

  • Learn about our miRNA assays
    • Circulating assay flow
      • Whitepaper: see the data
        • Choosing a miRNA assay
          • FAQs
            • FirePlex® particle tech
              • FirePlex® publications
                • FirePlex® analysis software
                • Benefits of our assays
                  • What do I need?
                    • Panels for your research

                      ​​Find out how FirePlex particle technology works with your flow cytometer to allow highly sensitive, true multiplexed analysis.

                      Our multiplex miRNA assays allow simultaneous measurement of up to 68 target miRNAs in a single well, across many individual samples. At the heart of these assays is innovative FirePlex particle technology that provides this multiplexing ability and allows assay readout on a standard flow cytometer.


                      FirePlex particles

                      ​Particles are made from a porous bio-inert hydrogel that allows target capture throughout the 3D volume. Compared with the leading bead-based platform, FirePlex particle technology provides >100x more binding sites per particle. For nucleic acid capture and detection, this provides an additional two logs of dynamic range and ~75x increase in sensitivity.

                      Particles contain three distinct functional regions each separated from the other by inert spacer regions. The central analyte quantification region contains probes that capture target miRNAs. The two end regions function as two halves of a barcode to distinguish different particles.




                      End regions encode particles

                      • Contain differing fluorescence intensities to create a fluorescent barcode
                      • In a given panel, all particles that capture a particular miRNA contain the same code



                      ​Central analyte quantification region

                      • Contains probes to capture a specific target miRNA

                      Particle structure. Distinct regions capture target miRNAs and encode particles.


                      How are particles read by the flow cytometer?

                      As particles pass through a flow cytometer they appear as a series of three cells. By separating the target quantification region from the particle encoding information, we reduce cross talk between channels and eliminate the need for compensation.

                      The flow cytometer and FirePlex Analysis Workbench use information from the three regions to generate abundance data for each target miRNA:

                      • Particle presence is triggered in the green channel. Events from all three regions are detected in the green channel to signify the presence of a particle.
                      • Fluorescence intensity in the central region determines miRNA quantity. The flow cytometer detects a fluorescent signal in the red channel from the center of the of the particle. This comes from captured and labeled target miRNA and is proportional to the amount of miRNA present in the sample.
                      • A fluorescent code to distinguish different miRNAs. By containing two end regions of varying fluorescence intensity, a particle code is generated in the yellow channel. Up to 70 codes are possible, and in every panel each miRNA is randomly assigned one of those codes so that data from a mixture of particles can be attributed to individual target miRNAs.
                      • Software to stitch all the data together. After acquisition, the FirePlex Analysis Workbench software takes events from the three regions of the particles and stitches them back together into a single event, giving you simply the abundance data for each target miRNA.
                      Particle presence is triggered in the green channel. Events from all three regions are detected in the green channel to signify the presence of a particle.

                      Fluorescence intensity in the central region determines miRNA quantity. The flow cytometer detects a fluorescent signal in the red channel from the center of the particle. This comes from labeled target miRNA captured by the particle, and is proportional to the amount of miRNA present in the sample.

                      A fluorescent code to distinguish different miRNAs. By containing two end regions of varying fluorescence intensity, a particle code is generated in the yellow channel. Up to 70 codes are possible, and in every panel each miRNA is randomly assigned one of those codes so that data from a mixture of particles can be attributed to individual target miRNAs.

                      Software to stitch all the data together. After acquisition, the Firefly Analysis Workbench software takes events from the three regions of the particles and stitches them back together into a single event, giving you simply the abundance data for each target miRNA.


                      True multiplexing ability

                      FirePlex particle technology allows true multiplexing; the ability to measure multiple miRNAs in the same well, without physical splitting of the sample. The particles enable this by containing the particle code to identify individual miRNA species and a separate analyte quantification region.

                      True multiplexing. Multiple miRNAs are detected within a single well.

                      A mixture of particles—each specific to a particular target miRNA—is added to each well to capture up to 68 target miRNAs. The FirePlex Analysis Workbench software then differentiates between miRNAs using the particle code and combines this information with the signal from the central analyte quantification region to provide quantification data for individual miRNAs.

                      The particle mix added to each well contains on average 20 particles specific for each miRNA. This gives you 20 reads for every miRNA you measure and a maximum of 1,360 data points per well.

                      • Ready to get going? Select and order your miRNA assay now.
                      • Have specific requirements? Design a custom panel.



                      通过邮件收到学术资源和促销信息 注册
                      研究类别(A-Z)
                      • 肿瘤研究
                      • 心血管研究
                      • 细胞生物学
                      • 发育生物学
                      • 染色质及细胞核信号转导研究
                      • 免疫学
                      • 代谢
                      • 微生物学
                      • 神经生物学
                      • 信号转导研究
                      • 干细胞研究
                      产品种类(A-Z)
                      • 一抗
                      • 二抗
                      • 生化试剂
                      • 亚类对照
                      • 流式细胞仪多色选择器
                      • 试剂盒
                      • 内参对照
                      • 细胞裂解液
                      • 多肽
                      • 蛋白酶
                      • 组织切片
                      • 蛋白标签及细胞标记物
                      • 相关试剂及工具
                      帮助与支持
                      • 技术支持
                      • 技术FAQ
                      • 实验步骤与建议
                      • 购买FAQ
                      • RabMAb 产品
                      • 培训课程
                      • 按靶标浏览
                      企业
                      • 企业资讯
                      • 投资者关系
                      • 公司新闻
                      • 招贤纳士
                      • 关于我们
                      活动通知
                      • 商品展览
                      • 会议
                      全球网站
                      • abcam.com
                      • abcam.co.jp
                      QR code for Weechat

                      微信扫码 关注我们

                      沪ICP备14046373号-1

                      沪公网安备 31011502007413号

                      • 销售条款
                      • 网站使用条款
                      • Cookie政策
                      • 隐私权政策
                      • 法律
                      © 1998-2021 艾博抗(上海)贸易有限公司版权所有