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The analysis of cell viability, cytotoxicity, cell cycle state, cell proliferation, and cell death are critical to most cell-based studies.
Understand your options, and choose the right assay to fit your experimental needs.
Or read this online version of the guide.
Cell viability assays that measure the rates of cellular metabolism/enzyme activity
Including MTT, MTS, resazurin and similar assays, mitochondrial membrane potential-dependent dyes, esterase cleaved dyes, ATP and ADP assays, and assays that measure glycolytic flux and oxygen consumption.
Cytotoxicity assays that test for cytolysis/membrane leakage
Including LDH enzyme leakage assays, and membrane impermeable dyes like 7-AAD.
Cell proliferation and cell cycle assays
Including assays using dye dilution, BrdU/EdU and DNA staining dyes. These are used to monitor the growth of a cell population, detect generations of daughter cells, or analyze the cell cycle state of a cell population. You can use also these tests to assay cell viability.
Apoptosis/cell death analysis
Such as Annexin V, TUNEL, and caspase assays.
Remember: no one method gives a perfect view of cell viability, proliferation, or cell death. It is almost always most effective to combine several different methods.