存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab74030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Can be blocked with Human Kindlin 2 peptide (ab74029).|
功能Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
组织特异性Ubiquitous. Found in numerous tumor tissues.
序列相似性Belongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain.
结构域The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
细胞定位Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
- Information by UniProt
- FLJ34213 antibody
- FLJ44462 antibody
- UNC112 antibody
All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml
Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 8 minutes
Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 78kDa; Kindlin 2
The kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.
ab74030 staining Kindlin 2 in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using Triton, then incubated with ab74030 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a Cy3-AffiniPure F(ab')2 fragment goat anti-rabbit monoclonal, used at a 1/200 dilution.
This product has been referenced in:
- Artym VV et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol 208:331-50 (2015). Read more (PubMed: 25646088) »
- Wu C et al. Kindlin-2 controls TGF-ß signalling and Sox9 expression to regulate chondrogenesis. Nat Commun 6:7531 (2015). IHC . Read more (PubMed: 26151572) »