Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (Forebrain)
Total protein in input
20000 µg
Specification
Forebrain
Immuno-precipitation step
Protein A
Other product details
Dilution
1/4000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: HBS, containing 0.5% Triton X-100
Additional data
Additional Notes
Lysys buffer was 10mM HEPES, pH, 7.4, containing 145mM NaCl, 1mM EGTA, 0.1mM MgCl2, 1% (v/v) Triton X-100 [(10 micrograms/ml) benzamidine, soybean trypsin inhibitor, chicken egg trypsin inhibitor, bacitracin and phenylmethyl sulfonic acid. Proteins were transferred to nitrocellulose at 14V for 16h at rt.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Kieran Brickley
Verified customer
提交于 May 06 2009