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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Microtubules

Anti-KIF5A抗体(ab5628)

  • Datasheet
Reviews (3)Q&A (1)References (10)

Product price, shipping and contact information

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Western blot - Anti-KIF5A antibody (ab5628)
  • Western blot - Anti-KIF5A antibody (ab5628)
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)

Key features and details

  • Rabbit polyclonal to KIF5A
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-KIF5A抗体
    参阅全部 KIF5A 一抗
  • 描述

    兔多克隆抗体to KIF5A
  • 宿主

    Rabbit
  • 特异性

    This antibody is specific for KIF5A and does not detect other kinesin isotypes.

  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse KIF5A aa 1007-1027.
    Sequence:

    CGYEAEDQAKLFPLHQETAAS


    (Peptide available as ab41781)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

     This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab5628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).

靶标

  • 功能

    Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • 组织特异性

    Distributed throughout the CNS but is highly enriched in subsets of neurons.
  • 疾病相关

    Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • 序列相似性

    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • 结构域

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • 细胞定位

    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Target information above from: UniProt accession Q12840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 511273 Cow
    • Entrez Gene: 3798 Human
    • Entrez Gene: 16572 Mouse
    • Omim: 602821 Human
    • SwissProt: Q12840 Human
    • SwissProt: P33175 Mouse
    • Unigene: 151219 Human
    • Unigene: 30355 Mouse
    • 别名

      • D12S1889 antibody
      • KIF 5A antibody
      • Kif5a antibody
      • KIF5A_HUMAN antibody
      • Kinesin family member 5A antibody
      • Kinesin heavy chain isoform 5A antibody
      • Kinesin Heavy Chain Neuron Specific antibody
      • Kinesin heavy chain neuron-specific 1 antibody
      • MY050 antibody
      • Neuronal kinesin heavy chain antibody
      • NKHC 1 antibody
      • NKHC antibody
      • SPG 10 antibody
      see all

    图片

    • Western blot - Anti-KIF5A antibody (ab5628)
      Western blot - Anti-KIF5A antibody (ab5628)
      Anti-KIF5A antibody (ab5628) + mouse neuronal cell extract

      Secondary
      HRP-conjugated anti rabbit antibody

      Predicted band size: 117 kDa



      Shows a Western blot of KIF5A on mouse neuronal cell extract using ab5628.

    • Western blot - Anti-KIF5A antibody (ab5628)
      Western blot - Anti-KIF5A antibody (ab5628)
      Anti-KIF5A antibody (ab5628) + human retinal extract

      Secondary
      HRP-conjugated anti-rabbit antibody

      Predicted band size: 117 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)
      Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)
      ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (10)

    发表研究结果有使用 ab5628?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5628 被引用在 10 文献中.

    • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
    • Shi L  et al. Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 family member calcyon. Mol Biol Cell 29:2055-2068 (2018). PubMed: 29949458
    • Kamata H  et al. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis. Sci Rep 7:4556 (2017). WB . PubMed: 28676645
    • Blakeslee WW  et al. Class I HDACs control a JIP1-dependent pathway for kinesin-microtubule binding in cardiomyocytes. J Mol Cell Cardiol 112:74-82 (2017). WB . PubMed: 28886967
    • Du W  et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell 37:326-336 (2016). PubMed: 27219061
    • Roche SL  et al. Loss of glial neurofascin155 delays developmental synapse elimination at the neuromuscular junction. J Neurosci 34:12904-18 (2014). Mouse . PubMed: 25232125
    • Cartelli D  et al. Microtubule alterations occur early in experimental parkinsonism and the microtubule stabilizer epothilone D is neuroprotective. Sci Rep 3:1837 (2013). WB ; Mouse . PubMed: 23670541
    • Astanina K & Jacob R KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Cell Mol Life Sci 67:1331-42 (2010). IP ; Dog . PubMed: 20094756
    • DeBoer SR  et al. Conventional kinesin holoenzymes are composed of heavy and light chain homodimers. Biochemistry 47:4535-43 (2008). PubMed: 18361505
    • Brickley K  et al. GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin. J Biol Chem 280:14723-32 (2005). PubMed: 15644324

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    1-4 of 4 Abreviews or Q&A

    Question

    Dear Abcam technical team, I want to order proteins from your website, but there are some questions before order. (1) I want to experiment with conventional kinesin heavy chain, KIF5. But there are three different members of kinesin family 5. KIF5A, KIF5B and KIF5C. I know the function of each member, A and C involves in neuronal transport otherwise B involves in inner cell transport. But is there any structural difference between three members? If is, I have to choose specific member to experiment. (2) There are two types in KIF5A and three types in KIF5B. It seems that each has different domain, so I wonder there are functional or structural difference between types. Also, your company has two different types of KIF5C. One is just (938-957) domain and the other is His-tagged protein. His tagged kinesin has higher protein purity. Is there any reason to choose His tagged KIF5C except higher purity? (3) Is antibody that has specificity of my protein (e.g. KIF5A --> Anti-kinesin 5A antibody) attach to specific domain (like C-terminal) or it is just randomly attached? For my experiment, it is good for antibody to attach to C-terminal. In other words, is antibody has C-terminal specificity? (4) Do you sell biotinylated antibody for kinesin? I want antibody that has biotin coating but your website has no option or products for biotin coating. If you cannot understand my questions, please let me know which parts you cannot understand. Then I will send the more specific explanation for my questions. From

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    Abcam community

    Verified customer

    Asked on Oct 11 2011

    Answer

    Thank you for contacting us and your interest in our products. 1.) Indeed, there is structural differences between those members. The sequences of the three members are not the same. I have made an alignment where you can see these differences. Please refer to the following link to see these differences: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111011-085702-0894-35538860-oy To check out further parameters of these proteins, I can recommend to browse either the expasy site (http://web.expasy.org/ or http://web.expasy.org/protparam/) as well as one of the Bioinformatics institutes homepages, for example the EBI, http://www.ebi.ac.uk/. There are different Tools to predict the protein structures. 2.) We do have currently only one KIF5 protein in our catalogue, and this is the ab91695 Kinesin 5C protein (Tagged-His Tag) which is not a full length protein but a recombinant fragment corresponding to amino acids 500-957 of Human Kinesin 5C. Click here (or use the following: https://www.abcam.com/Human-Kinesin-5C-protein-fragment-ab91695.html). All the other products are indeed peptides and do correspond to the short amino acid sequence of the numbers in brackets. These peptides are too short to be able to have a function similar to the whole protein, however they can be used for blocking studies with the corresponding antibody. For example ab41784 is a peptide corresponding the sequence 938 to 957 of human KIF5C. Similarly for the other products: KIF5A: - peptide amino acid 1019-1032 is ab23133 and - peptide amino acid 1007-1027 is ab41781 KIF5B - peptide amino acid 376-396 is ab41782 - peptide amino acid 951-963 is ab23262 - peptide ab100817, a few amino acids derived from the sequence between amino acid 350 to 450 3.) Each antibody is different. I have therefore put together the following list: KIF5A, we do have two antibodies: -ab5628 is a polyclonal antibody directed against the C terminus of KIF5A, recognizing the sequences of amino acids 1007 to 1027 of KIF5A. This antibody has also been tested against the KIF5A protein of different species (human, mouse, rat and cow) as well as in immunocytochemistry, immunoprecipitation and Western blot. Click here (or use the following: https://www.abcam.com/KIF5A-antibody-ab5628.html). -ab5958 which is a polyclonal antibody directed against the C terminal sequence (amino acids 1019 - 1032 of KIF5A). This antibody has however not been well characterized as yet, as you see it is one of our Fasttrack antibodies. Please let me know if you would like to know more about this. Click here (or use the following: https://www.abcam.com/KIF5A-antibody-ab5958.html). KIF5B we have 7 antibodies currently: two of them are against a region in the C terminal: ab15705, corresponding to amino acids 951-963 Click here (or use the following: https://www.abcam.com/Kinesin-5B-antibody-ab15705.html). ab97762, corresponding to amino acids 901 - 963 Click here (or use the following: https://www.abcam.com/KIF5B-antibody-ab97762.html). The other KIF5B antibodies are either N terminal (ab42492, immunogen amino acids 7-56), from the central region (ab5629, immunogen amino acids 376-396 and ab91033 region derived from amino acids 350 - 450) or are mouse monoclonal antibodies for which we do not know the epitope (ab110155 and ab11262) KIF5C we only have one antibody: ab5630 which has been raised against a C terminal sequence as well, the amino acids 938 - 957 of the human KIF5C. Click here (or use the following: https://www.abcam.com/KIF5C-antibody-ab5630.html). 4.) Indeed we do currently not have one of these antibodies in biotinylated form. We do have however the Easylink conjugation kits, which are especially designed for easy conjugation of antibodies. We do have such kits also to perform biotin conjugation. You can find more information in this regards under the following website: www.abcam.com/easylink Again, please let me know if you wish more information about this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Oct 11 2011

    Western blot abreview for Anti-Kinesin 5A antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
    Loading amount
    10 µg
    Specification
    Human SH-SY5Y neuroblastoma cells
    Gel Running Conditions
    Reduced Denaturing (7.5 %)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Mrs. Adelheid Hanes

    Verified customer

    提交于 Nov 15 2010

    Immunoprecipitation abreview for Anti-Kinesin 5A antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (Forebrain)
    Total protein in input
    20000 µg
    Specification
    Forebrain
    Immuno-precipitation step
    Protein A
    Read More

    Dr. Kieran Brickley

    Verified customer

    提交于 May 06 2009

    Western blot abreview for Anti-KIF5A antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat whole brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Rat whole brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 15 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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