重组Anti-KIF4A/KIF4抗体[EPR5459] (ab124903)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5459] to KIF4A/KIF4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-KIF4A/KIF4抗体[EPR5459]
参阅全部 KIF4A/KIF4 一抗 -
描述
兔单克隆抗体[EPR5459] to KIF4A/KIF4 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human KIF4A/KIF4 aa 50-150. The exact sequence is proprietary.
-
阳性对照
- Mouse thymus tissue lysate; HeLa cell lysate; 293T cell lysate; 293 cells
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.52 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5459 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
|
说明 |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa). |
靶标
-
功能
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
组织特异性
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
序列相似性
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
细胞定位
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
-
数据库链接
- Entrez Gene: 24137 Human
- Entrez Gene: 16571 Mouse
- Entrez Gene: 84393 Rat
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- SwissProt: P33174 Mouse
- Unigene: 648326 Human
-
别名
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
图片
-
All lanes : Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 140 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of permeablized 293 cells using ab124903 at 1/10 dilution (red) or a rabbit IgG negative control (green).
数据表及文件
-
SDS download
-
Datasheet download
文献 (12)
ab124903 被引用在 12 文献中.
- Schneider MWG et al. A mitotic chromatin phase transition prevents perforation by microtubules. Nature 609:183-190 (2022). PubMed: 35922507
- Sun X et al. KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma. Thorac Cancer 12:512-524 (2021). PubMed: 33350074
- Song Y et al. Identification of KIF4A and its effect on the progression of lung adenocarcinoma based on the bioinformatics analysis. Biosci Rep 41:N/A (2021). PubMed: 33398330
- Zhang DY et al. KIF4A as a novel prognostic biomarker in cholangiocarcinoma. Medicine (Baltimore) 100:e26130 (2021). PubMed: 34032761
- Cui X et al. Increased expression of KPNA2 predicts unfavorable prognosis in ovarian cancer patients, possibly by targeting KIF4A signaling. J Ovarian Res 14:71 (2021). PubMed: 34034774